创伤性脑损伤大鼠P300检测与海马MicroRNA的表达变化.docVIP

  创伤性脑损伤大鼠 P300 检测与海马 MicroRNA 的表达变化# 孙婷怡，刘子龙，刘良** 5 10 15 20 25 30 35 40 （华中科技大学同济医学院法医学系，武汉 430030） 摘要：目的 探索创伤性脑损伤后大鼠海马组织 microRNA 的表达变化与认知功能障碍的相 关性。方法 25 只 SD 大鼠随机分为假手术组、伤后 1h 组、伤后 1d 组、伤后 3d 组、伤后 5d 组，采用 Feeney 自由落体法建立创伤性脑损伤动物模型。各组大鼠于伤后相应时间点行 事件相关电位 P300 检测，随后断头处死大鼠，应用基因芯片技术分析海马组织 microRNA 的表达情况，筛选特异性表达的 microRNA。结果 各组大鼠伤后 P300 的潜伏期延长，波幅 下降，伤后 1d 组潜伏期延长达到顶峰。基因芯片分析结果表明，与假手术组相比，伤后 1h 组、1d 组、3d 组、5d 组 microRNA 表达谱具有特异性。其中 miR-21、miR-16、let-7b 的表 达与 P300 潜伏期变化存在显著相关，伤后 1d 为差异性表达的关键时间点。结论 大鼠创伤 性脑损伤后海马 microRNA 表达与 P300 变化存在一定相关性，提示 microRNA 可能在创伤 性脑损伤后发生的认知功能障碍中发挥调控作用，有望为脑损伤后认知功能的法医学鉴定提 供新的思路。 关键词：法医临床学；创伤性脑损伤；microRNA；听觉事件相关电位；海马 The Relationship between MicroRNA Profiling of Rat Hippocampus and the P300 Event-related Potential following Traumatic Brain Injury SUN Tingyi, LIU Zilong, LIU Liang (Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, WuHan 430030) Abstract: Objective To explore the correlation between microRNA profiling of rat hippocampus and cognitive function disorder with traumatic brain injury. Methods Totally 25 SD rats were randomly divided into sham-operated group and experimental group including 1h group, 1d group, 3d group and 5d group. The brain injury groups were prepared by improved Feeneys free falling method. The event-related potential P300 was examined at each injury time point, and subsequently, all rats were killed rapidly. We characterize the microRNA expression profile in rat hippocampus using the Significance Analysis of Microarrays analysis to identify differentially expressed microRNAs. Results The latencies were significantly prolonged and the amplitudes were reduced in experimental groups compared with that of P300 in sham group, which the latency reached its max value at 1 day post injury. Through comparison between TBI groups and sham-operated group, the microRNA microarray analysis results indicated that up- or down-regulation of the differentially-expressed microRNAs in