牛磺酸对大鼠肢体缺血再灌注后肝脏损伤的保护效应论文.docVIP

　　牛磺酸对大鼠肢体缺血再灌注后肝脏损伤的保护效应论文 王银环，董淑云，张娜，赵利军，李宏杰，孔小燕，刘丽华，张连元 【关键词】 再灌注损伤；肝功能；四肢；牛磺酸 Protective effect of taurine against liver injury after limb ischemiareperfusion in rats 【Abstract】 AIM: To observe the liver injury and investigate the protective effect of taurine on the liver function after limb ischemiareperfusion. METHODS: Thirty DA, AST, ALT and SOD in plasma easured after 4 h limb ischemia and 4 h reperfusion in each group. XOD, MDA, ROS, MPO and ［Ca2+］ in the liver tissues and GSHPX, ［Ca2+］ in the liver mitochondria ol/L vs (1.27±0.21) nmol/L］ , ALT ［(78.40±6.45) nkat/L vs (20.50±3.70) nkat/L］,.freela increased, but SOD ［(1.30±0.15) μkat/L vs (1.80±0.16) μkat/L ］ reduced, XOD ［(104.69±12.34) μkat/kg vs (59.01±10.50) μkat/kg］, MDA ［(2.66±0.08) nmol/L vs (1.29±0.14) nmol/L］, ROS ［(771.65±100.69) μkat/L vs (606.45±52.01) μkat/L］, MPO (0.47±0.04 vs 0.28±0.06), ［Ca2+］［(0.248±0.050) mmol/L vs (0.123±0.014) mmol/L］ in the liver tissues increased, GSHPx ［(20.34±4.67) nkat/L vs (31.17±11.50) nkat/L］ in the liver mitochondria mol/L vs (0.14±0.03) mmol/L］ increased; In TR group pared ol/L］, ALT ［(64.40±9.05) nkat/L］, AST ［(38.70±8.10) nkat/L］ a of TR group increased; the values of XOD ［(94.19±13.50) μkat/L］, MDA ［(1.67±0.12) nmol/L］, ROS ［(710.81±55.34) μkat/ L］, MPO (0.36±0.04), ［Ca2+］［ (0.192±0.426) mmol/L］ in the liver tissues decreased, the values of GSHPx［(22.50±3.17) nkat/L］ in the liver mitochondria of TR group obviously increased, but ［Ca2+］ ［(0.31±0.06) mmol/L］ reduced. CONCLUSION: The taurine could protect the liver function of rats after limb ischemiareperfusion. 【Keyities; taurine 【摘要】目的： 观察大鼠肢体缺血再灌注后肝脏的损伤性变化，以及牛磺酸对肝脏损伤性变化的保护效应，探讨牛磺酸对大鼠肢体缺血再灌注后肝脏功能保护作用的可能机制. 方法： 实验用DA, AST, ALT和SOD的变化；观察肝组织XOD, MDA, ROS, MPO和Ca2+的变化；观察肝线粒体GSHPX和Ca2+的变化. 结果： 单纯肢体缺血再灌注组大鼠血浆中LDH ［(190.16±13.36) μkat/L］, XOD ［(758.82±151.53) nkat/L］, MDA ［(5.19±0.67) nmol/L］, ALT ［(78.40±6.45) nkat/L］, AST ［(47.70±4.47) nkat/L］ 等较正常对照组血浆中LDH［(122.39±14.87) μkat/L］, XOD［(543.61±43.51) μkat/kg