金银花提取液抗氧化活性研究.PDFVIP

※基础研究 食品科学 2009, Vol. 30, No. 21 63 罗 磊，郭晓园 (河南科技大学食品与生物工程学院，河南洛阳 471003) 采用三价铁离子还原法、羟自由基( ·OH)体系和二苯代苦味酰基自由基(DPPH ·)体系对金银花提取液和 绿原酸标准品抗氧化活性进行研究，以抗坏血酸为对照。结果表明：金银花提取液清除·OH 和DPPH ·的能力 最强，对·OH 半数抑制浓度(IC50) 为0.0273mg/ml ，提取液的绿原酸浓度为0.006mg/ml 时对DPPH ·的抑制率达 80%；而绿原酸标准品的还原能力要大于金银花提取液(2.66 倍)和抗坏血酸(2.42 倍)；绿原酸为金银花提取液抗氧化 作用的主要物质。 金银花；绿原酸；抗氧化活性 Antioxidant Activity of Honeysuckle Extract LUO Lei ，GUO Xiao-yuan (Food and Bioengineering Department, Henan University of Science and Technology, Luoyang 471003, China) Abstract：The antioxidant activities of honeysuckle extract and chlorogenic acid were evaluated by ferric ion reducing antioxidant power (FRAP) assay, hydroxyl free radical array and DPPH free radical assay using vitamin C as the reference. Results showed that honeysuckle extract had strong scavenging capacity for hydroxyl and DPPH free radicals. The IC50 of honeysuckle extract for scavenging hydroxyl free radicals was 0.0273 mg/ml. The scavenging rate of honeysuckle extract for DPPH free radicals was 80% when its chlorogenic acid content was 0.006 mg/ml. Meanwhile, the reducing power of chlorogenic acid standard was 2.66- fold and 2.42-fold stronger than that of honeysuckle extract and vitamin C, respectively. Therefore, chlorogenic acid is the major antioxidant component in honeysuckle extract. Key words ：honeysuckle ；chlorogenic acid ；antioxidant activity 中图分类号：Q949.781.2 文献标识码：A 文章编号：1002-6630(2009)21-0063-03 金银花又名忍冬(Lonicera japonica Thurb) ，隶属 品能否作为良好的电子供体，其还原能力与抗氧化活性 忍冬科(Caprifoliaceae) ，忍冬属(Lonicera )植物，简称银 之间有显著的相关性[3-6] 。本研究采用典型的体外抗氧化 花。在我国资源丰富，主产地为山东、河南。药理 体系，以抗坏血酸为对照，从多种方面评价金银花提 研究表明金银花具有