舒心饮对急性心肌缺血鼠血小板活化的影响.pdfVIP

·1122· 中医杂志2007年第48卷第 l2期 Journal of Traditional Chinese Medicine，2007，Vo1．48，No．12 舒心饮对急性心肌缺血鼠血小板活化的影响 何 燕 陈竞纬 孙丽华 林种香 (上海中医药大学附属龙华医院心内科，上海市宛乎南路725号，200032) [摘 要] 目的 研究舒心饮对急性心肌缺血鼠血小板活化的影响，以阐明舒心饮治疗冠心病的可能机制。方法 建立大鼠急性 心肌缺血模型和小鼠急性心肌缺血再灌注模型。雄性SD大鼠和ICR小鼠各 51只，随机各分 5组，包括假手术组，模型组，舒心饮 大、小剂量组，抵克力得组。连续灌胃3O天后造模、采血。运用流式细胞术，单克隆抗体检测小鼠血小板膜糖蛋白P一选择素(cD P)；运用放射免疫法等检测大鼠血小板聚集率(PAR)、纤维蛋白原(Fg)、血栓素B (TXB )和6一酮一前列腺素F， (6一keto—PGF ) 及其比值。 结果 舒心饮能抑制急性心肌缺血再灌注小鼠CD。 P的高表达，降低急性心肌缺血大鼠的PAR、Fg和TXB ／6一keto— PGF 的比值，与对照药抵克力得比较差异无显著性意义。结论 舒心饮可有效抑制急性心肌缺血鼠血小板活化，其防治冠心病的 作用与其抗血小板活化机制有关。 [关键词] 舒心饮；冠心病；急性心肌缺血；血小板活化 Influence of Herbal Decoction Shuxin Yin on Platelet Activation in Rats and Mice with Acute Myocardial Ischemia HE Yan，CHEN Jing—wei，SUN Li—hua，et a1． (Longhua Hospital of Shanghai University of Traditional Chinese Medicine，Shanghai 200032) ABSTRACT Objective To study the influence of herbal decoction Shuxin Yin(SXY)on platelet activation of rats and mice with a— cute myocardial ischemia in order to explain its possible mechanism for treating coronary heart disease．Methods The rat model with acute myocardial ischemia and the mice model with acute myocardial ischemia and reperfusion were established．The male SD rats and ICR mice，5 1 each，were randomized into 5 groups respectively：sham operation group，model group，low dose SXY group。high dose SXY group，ticlopidine group．Made models and collected blood Sample after 30 days gastric infusion in succession．The flow cytome— try technique and the monoclonal antibody examination were adopted to test the mice platelet glycoprotein CD62 P；the radioim— munoassay was used to test rat platelet aggregation rate(PAR)，fibrinogen(Fg)，thromboxane B2(TXB2)and 6-keto—PGF1 and their ratio．Results Shuxin Yin can，inhibit the high expression of CDg2P of mice with acute myocardial ischemia and reperfusion，and de- crease PAR，Fg and TXB2／6一keto—PGFl of rats w