补肾抗衰汤对氧化应激所致的秀丽隐杆线虫生育缺陷的影响论文.docVIP

　　补肾抗衰汤对氧化应激所致的秀丽隐杆线虫生育缺陷的影响论文 曹新国, 侯莉莉, 陈军霞, 陆勤 【摘要】 目的：探讨补肾抗衰汤对秀丽隐杆线虫因氧化应激诱导所致生育缺陷的作用。方法：将终浓度为0.33 g/mL的补肾抗衰汤按25%、50%、75%和100%梯度稀释后对L4期线虫培养。应激方式采用紫外线照射［20 J/(m2·min)］、热休克（36 ℃, 2 h）和百草枯(2 mmol/L, 2 h)处理。通过子宫内卵数、卵的大小和传代时间来评价秀丽隐杆线虫的生育能力。结果：野生型N2线虫经过紫外线照射、热休克和百草枯处理后，子宫内卵数、卵的大小均显著减少.freelev1突变体线虫受损的生育能力。 结论：氧化应激对生育能力有负面影响，高浓度补肾抗衰汤可明显逆转氧化应激所致的线虫生育缺陷。 【关键词】 补肾抗衰汤; 氧化应激; 生育; 线虫 The reproductive successes of animals and humans depend on a series of careful neuroendocrine events, and the reproduction failure ing for any one of these events is disrupted［1］. Under such conditions, animal reproduction appears to be the most vulnerable［1］. For example, ent, and impaired embryonic development［2］. Stress can be defined as an event (physical,.freelental, psychological, etc.) eostasis of the animals. In mammals, stress impacts on the reproductive axis at the hypothalamus and the pituitary gland［3］. Various stressful conditions have adverse effects on reproduction of animals, and the stress conditions include environmental factors (i.e., temperature, humidity, metals and irradiation), physical factors (i.e., transport, shearing, restraint and strenuous exercise), metabolic factors (i.e., insulininduced hypoglycaemia), immunological factors (i.e., infection and endotoxins administration), and psychological factors (i.e., isolation, social interactions and mental arithmetic tasks)［15］. Caenorhabditis elegans, a classical model organism, is a free living nematode , specialized muscles, and digestive and reproductive systems［6］. The reproductive process has been investigated extensively in C.elegans［79］. C.elegans hermaphrodites are selffertile, and their reproductive process is modulated by diverse environmental cues［79］. Among these environmental cues, environmental stress can affect the reproductive process of nematodes significantly. The data from Coohill et al［10］ shoent and fertility. The reproductive process can also be altered by heat stress in nematodes［11］. Electro