核桃与铁核桃种间关系的RAPD鉴定.pdfVIP

第25卷 第4期 四川农业大学学报 V()l 25 No．4 2007年 l2月 Journal of Sichuan Agricultural University Dec．2007 Identification tof Relationship between Juglans regia L． and ．si gillata Dode by RAPD Markers CHEN Lian8一huo，HU Ting—xin8 ，ZHANG Fan (College of Forestry and Horticulture，Sichuan Agricultural University，Yaan 625014，Sichuan，China) Abstract：Random amplified polymorphic DNA(RAPD)was applied to detect the relationship at the species level between Jugla,~s regia L．and J．sigillata Dode．Both J．regia DNA—pool and J．sigilla— ta DNA—pool were set up by using 8 J．regia samples and 8 J．sigillata samples，respectively．327 primers were screened，of which 10 polymorphic primers were identified．Two DNA—pools and all in— dividuals were verified by using the 10 polymorphic primers three times．A 250 bp DNA band was am— plified only from primer in D6 J．regia DNA—pool and 8 corresponding samples of J．regia，but absent in both J．sigillata DNA—pool and 8 corresponding sam ples of J．sigillata．The results tentatively showed that D6— 250 was a special molecular marker related to J．regia and J．sigillata at the species leve1．This marker can provide some basis for the relationship identification of germplasm resources of the two species． Key words：Jugla,u regia L．；J．sigillata Dode；RAPD；DNA—Pool · 研究简报· 核桃与铁核桃种间关系的RAPD鉴定 陈良华，胡庭兴 ，张 帆 (四川农业大学林学园艺学院，四川雅安 6250114) 摘要：运用RAPD技术对核桃与铁核桃特征性条带进行标记。分别利用8个核桃样品和8个铁核桃样品构建2个 物种的基因池，用327个随机引物，选出10个多态性引物，进一步用这l0个引物进行单株确认，发现只有引物D6 在核桃类群的8个样品中扩增出一条250bp的特异带，而在铁核桃类群的8个样品中无此特异带。初步分析认为， D6—250可能是核桃与铁核桃之间的特异性分子标记，该结果可为核桃和铁核桃种质鉴定提供一定的理论依据。 关键词：核桃；铁核桃；RAPD；基因池 中图分类号：S664．1 文献标识码：A 文章编号：1000—2650(2007)04—0513—04 核桃(Jugla7lS regia L．)和铁核桃(J．si