转录因子PAX5在儿童急性白血病细胞中的表达论文.docVIP

　　转录因子PAX5在儿童急性白血病细胞中的表达论文 .freelRNA的表达水平。结果表明：在NamalRNA相对表达量分别为2.35%和2.52%；而在T-和髓系细胞株中几乎不表达。在临床样本中B-ALL组比T-ALL组和AML组的PAX5 mRNA表达高（P=0.029和P=0.013）。T-ALL组和AML组的PAX5 mRNA表达水平均低于正常对照组，而T-ALL组与AML组之间的表达差异无显著意义。在B-ALL患儿中，PAX5 mRNA表达的个体差异很大。此外还发现，初发治疗前组和复发组的B-ALL患儿PAX5 mRNA表达高于化疗完全缓解组（P=0.011和P=0.006）。由于在CD19基因的启动子上有B细胞特异性激活蛋白的结合位点，研究发现在B-ALL中PAX5表达水平与同样用实时定量RT-PCR检测的CD19表达水平之间存在明显的相关性。结论：运用实时定量RT-PCR方法能快速准确地在B-ALL的临床标本中检测和定量PAX5基因的表达。研究中发现部分B-ALL中PAX5 mRNA表达明显升高，因此它可将其作为进一步研究B-ALL发病机理的另一个切入点。 【关键词】 转录因子 Expression of the Transcription Factor PAX5 in Childhood Acute Leukemic Cells Abstract To investigate transcription factor PAX5 expression characteristics in childhood acute leukemic cells，expression levels of PAX5 and CD19 mRNA in 6 hematological tumor cell lines and bone marroal children，58 de novo patients and 4 relapse acute leukemic children，including 39 cases of B-ALL，10 cases of T-ALL and 13 cases of AML，e RT-PCR. The results shoRNA expression levels alost not detectable in other T- and myeloid cell lines. Among clinical samples，expression of PAX5 mRNA in B-ALL al controls.The difference of PAX5 mRNA expression levels betRNA expression levels in children RNA expressions in de novo and relapse patients ission (P=0011 and P=0.006 respectively). As binding sites for B-cell specific activator protein have been identified in the promoter regions of CD19，the study found that in B-ALL，there e RT-PCR. It is concluded that PAX5 transcripts are readily detectable and quantifiable in clinical materials e RT-PCR. The strong PAX5 mRNA expression in some B-ALL can be considered to be particularly interesting for further analysis. Key ic cell；real-time RT-PCR PAX5基因是成对区PAX基因家族的成员，它编码的核反式作用因子——B细胞特异性激活蛋白（B-cell specific activator protein，BSAP）在发育的中枢神经系统、成人睾丸和B淋巴细胞中表达［1］。在B细胞发育过程中，PAX5基因的转录起始于Pro-B（Pre-B1）期，表达于Pre-B和成熟B细胞中，但在浆细胞中不表达［1，2］。BSAP通过与多种B细胞特异性基因（如CD19，VpreB，RAG2，mb-1）启动子或增强子上的BSAP结合位点结合，对这些基因的表达起着调控作用，因而BSAP在B细胞定向分化、发育、增殖、同型转换、免疫球蛋白分泌和终末分化阶段中发挥了重要的作用［1］。近年来研究表明，PAX