室温条件下油藏采出液微生物群落结构稳定性研究选编.docxVIP

室温条件下油藏采出液微生物群落结构稳定性研究*胡婧**吴晓玲宋永亭曹嫣镔汪卫东 高光军 孙刚正1中石化胜利油田石油工程技术研究院东营 257000摘要获得准确的油藏微生物群落信息是开展油藏微生物工业化应用的重要前提。高通量测序技术的应用极大地提高了油藏微生物群落结构解析的准确率和覆盖率，但油藏采出液在脱离油藏环境后，其中的微生物群落结构是否会在室内放置过程中发生改变；一个时间点的单次取样是否具有代表性的问题一直没有明确的结论。本研究针对以上两个问题，利用目前最先进的高通量测序技术，对胜利油田油藏采出液在脱离油藏环境后，室温放置5d内的微生物群落结构以及连续5d独立取样的同一油井水样间的微生物群落结构开展了相关研究。通过数据解析证实油藏采出液脱离油藏环境后，在室内放置0d-5d的过程中，细菌和古菌群落结构中的优势菌群没有发生明显改变，细菌的优势菌为热硫还原杆菌属（Thermodesulforhabdus）、无色杆菌属（Achromobacter）、沙雷氏菌（Serratia）未分类的细菌；古菌的优势菌为古生球菌属（Archaeoglobus）、产甲烷球菌属（Methanococcus）和甲烷鬃毛菌属(Methanosaeta)。虽然优势菌群结构具有稳定性，但细菌和古菌的多样性存在逐渐降低的趋势，其中古菌的多样性的变化比细菌更为明显。同时研究也证实同一口油井连续5d独立取样的样品间，细菌和古菌群落结构中的优势菌群也没有发生较大的变化，与室内放置样品的优势菌属一致，多样性分析同样发现油藏内不同时间点古菌的多样性的波动较细菌的大。就优势菌群来说，一次取样的油水井样品具有短时间的阶段代表性。以上研究成果解决了一直困扰本领域研究人员的疑问，为高通量测序技术在油藏微生物群落结构解析中的应用提供了重要的理论支撑. （图7表1参24）关键词高通量测序；油藏细菌群落；细菌古菌群落；群落结构稳定性CLCQ89Study on the stability of microbial community structure in reservoir water samples*HU Jing**, WU Xiaoling, Song Yongting, Cao Yanbin, Wang Weidong GAO GuangjunSun GangzhengResearch institute of petroleum engineering and technology, Shengli oilfield company, sinopec, Dongying 257000, ChinaAbstractMicrobial community in reservoir water samples is the biological basis forindustrial application. Although the application of high-throughput sequencing technology has greatly improved the accuracy and coverage of the microbial community structure, there are still have some questions. It is unclear if the microbial community structure in the reservoir samples will be changed after being separated from the reservoir environment and whether the single sampling is representative.In this study, the stability of the microbial community structure in the produced fluid of reservoir during placed indoor for 5 days and continuous independent sampling for 5 days was both analysed by high-throughput sequencing technologies.The study confirmed that there is no obvious change in the dominant microbial community structure but the diversity of bacteria and archaea decreased with the extension of i