番茄斑萎病毒属病毒检测技术研究进展_郑元仙.pdfVIP

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番茄斑萎病毒属病毒检测技术研究进展_郑元仙.pdf

番茄斑萎病毒属病毒检测技术研究进展_郑元仙

DO I :10.16211/j .issn.1004 -390x (n .2009.04.019 24   4                       Vo.l24 No.4 2009  7                  Ju.l2009 JournalofYunnanAgriculturalUniversity * 郑元仙, 刘雅婷** ( , 650201 :, 、 , , 。 。, , 、 、 、 。 :;;;;; :S432.41  :A  :1004-390 X(2009 04-0 607-07 AdvancesonDetectionTechniquesforTospovirus ZHENGYuan-xian, LIUYa-ting (CollegeofAgronomyandBiotechnology, YunnanAgriculturalUniversity, Kunming650201, China Abstrac:tGenusTospovirusbelongingtothefamilyBunyaviridaeisanmiportantplantvirusgenu.s Tospovirushaswiderangesofnaturalhostsandcaninfectagriculturalmiportantcropsandgardenplants andcausegreateconomiclossworldwide.Thehighlyeffectiveviralappraisalortheexaminationtech- nologyisoneofessentialfoundationsforpreventingandcontrollingplantviraldisease.Thu,sthead- vancesonthetechniquesandmethodstodetecttospoviruseswerereviewedinthispape.rThecharac- tersofthemethodscommonlyusedtotospovirusesdiagnosi,s suchasbiologicaltechnique,s electron microscop,e mimunologicalmethodsandmolecularbiologicaltechniquesandsoon, andtheirdirec- tionofdevelopmentandapplicationwereintroduced. Keyword:sTospoviru;sTomatospottedwiltviru;sbiologicaltechnique;s electronicmicroscope; mi- munologicalmethod;sPCR    (Tospovirus 2 G G。 1 2 (Bnuyaviridae, RNA(-ssRNA , 3 1, 1 , LRNA, MRNA [2] ——— (Tomatospottedwiltviru,s SRNA 。 [1] TSWV 。 Tospovirus Tospovirus, 、 , 80 ~110 nm, 1, ,

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