cAMP_PKA信号通路介导人脂肪干细胞成骨分化的体外试验_张磊.pdfVIP

·556 · 临床骨科杂志　JournaofCinicaOrthopaedics　2010 Oc;t13 (5) · · cAMP/PKA 1 2 1 3 1 1 张　磊 ,肖　扬, 吴震东, 陈　磊 ,应志豪, 陈子高 :　 (cAMP)/ A(PKA) (ADSCs)。 　 cAMP/PKA db-cAMP H-89, 、 PKA CREB。　db-cAMP PKA, CREB, ADSCs, PKA H-89 。　cAMP/PKA ADSCs。 :;;cAMP/PKA; :R336;R345　:A　:100 8-02 87 (2010 )05-0556-03 cAMP/PKAreguatesosteogenicdifferentiationofhumanadipose-derivedstemcesinvitro 　ZHANGLe,iXIAOYang, WUZhen-dong, CHENLe,iYINGZhi-hao, CHENZi-gao　(DeptofOr- thopaedic,sthe118 thHospitaofPLA, Wenzhou, Zhejiang　325000, China) Abstrac:tObjective　Toinvestigatetheeffectofcycicadenosinemonophosphate/proteinkaseA(cAMP/PKA)on osteogenicdifferentiationofhumanadipose-derivedstemces(hADSCs)invitro.Methods　PKAactivatordb-cAMP andinhibitorH-89 wereaddedtocuturemedium, andthechangeofosteogenicdifferentiationofhADSC,sPKAactiv-i tie,sphosphoryationoftranscriptionfactorCREBandgeneexpressionofosteogenesiswereobserved.Resuts　 db- cAMPincreasesedPKAactivityandpromotedphosphoryationofCREBandupreguatedgeneexpressionofosteogene- sisandenhancedosteogenesisofhADSC,swhietheseeffectcoudbedepressedbyH-89.Concusions　cAMP/PKA isakeysignapasswaytoinduceosteogenicdifferentiationofhADSC.s Keywords:adipose-derivedstemce;sosteogenesi;scycicadenosinemonophosphate/proteinkaseA;signatransduction 　　 (adipose-derivedstemce,sADSCs) ADSCs, ADSCs 、 [4-6]。 , , , 1　 , [1-3] 。 ADSCs 1.1　　Ⅰ、 , ADSCs (Dex)、 cAMP/PKA db-cAMP( , ADSCs Sigma, );DMEM、 。 A(PKA) (FBS, Gibco, ); , (ALP)(, , ADSCs :bs1535 R);RT-PCR、PepTagAssay , , (cAMP)/PKA ( Promega, /02 );