基于附子化学组分动态变化规律的附子配伍甘草最佳煎煮条件的研究.docVIP

基于化学组分动态变化的附子配伍甘草最佳煎煮条件的研究 秦素红，章津铭1，何宇新2，何瑶1，廖婉1，傅超美1*， （1. 成都中医药大学药学院，四川 成都，61）-甘草合煎液中总碱和三种双酯型生物碱含量以确定最佳煎煮条件，继而考察最佳煎煮工艺所得汤液的大鼠心脏毒性。结果：在0~90 min煎煮过程，附甘配伍前后其乌头总碱双酯型生物碱出现变化趋势，30 min两者综合的峰值；煎煮30 min的单附煎液和附甘合煎液-甘草配伍“减毒存性”的最佳煎煮条件为大火煮沸后小火微沸保持30 min。 关键词：附子；甘草；化学组分；动态变化；煎煮时间 Base on the Dynamic Change of Aconite Chemical Components to Study the Optimum Decoction Conditions of Compatibility of Aconite and Glycyrrhiza QIN Su-hong1，ZHANG Jin-ming1，HE Yu-xin2，HE Yao1，LIAO Wan1，FU Chao-mei1* （1. ChengDu University of TCM，SiChuan ChengDu，61Xihua University，SiChuan ChengDu，610039）Objective：Through the research on the Dynamic Change of Aconite Chemical Components of Aconite combine with Glycyrrhiza and Aconite during the decoction process, to determine the Optimum Decoction Conditions of Compatibility of Aconite and Glycyrrhiza. Method: After decocting for different time respectively, the samples of Aconite decoction and Decoction of Aconite combine with Glycyrrhiza were subsequently obtained for the measurement of the Aconite total alkaloids and three diester-alkaloids.accordingly, to determine the Optimum Decoction Conditions of Compatibility of Aconite and Glycyrrhiza.And then, the investigation on the rats cardiac toxic reaction of the Optimum Decoction is to further verificate the result. Results: In the 0-90 min decoction process, the Dynamic Change trend of the Aconite total alkaloids and three diester-alkaloids of Aconite decoction and Decoction of Aconite combine with Glycyrrhiza are different. In 30 min , Reaching to both peak value; Both Aconite decoction and Decoction of Aconite combine with Glycyrrhiza for 30 minutes have the cardiac toxic reaction effect on rats, but Decoction of Aconite combine with Glycyrrhiza is relative less. Conclusion: The Compatibility of Aconite and Glycyrrhiza has the effect of “reducing the toxicity and storing the efficacy of Aconite”, and the Optimum Decoction Conditions is boiling with huge conflagration and then boiling constant with s