喷射心肌打孔填充不同凝胶支架对犬心肌孔道保留-第三军医大学学报.DOC

喷射心肌打孔填充不同凝胶支架后心肌孔道演变规律的实验研究 周大燕，黄 晶，熊 鹿，郭 睿，吴 奇，容顺康，江永红，刘地川，邓昌明 （400010 重庆，重庆医科大学附属第二医院心血管内科） [摘要] 目的 观察喷射心肌打孔填充不同凝胶支架后犬心肌孔道的演变规律以及不同凝胶支架在心肌组织中的组织相容性。 方法 只体g，年龄1～2岁，随机分为agarose hydrogel，AH）组fibrin glue，FG）组chitosan hydrogel，CH）组取心肌组织行HE结果 结论；注射, 喷射；凝胶类；Trans Myocardial channels changes of transmyocardial jet revascularization with different hydrogel scaffolds filled the channels Zhou Dayan, Huang Jing, Xiong Lu, Guo Rui, Wu Qi，Rong Shunkang, Jiang Yonghong, Liu Dichuan, Deng Changming（Department of Cardiology，Second Affiliated Hospital of Chongqing University of Medical Sciences，Chongqing 400010, China） Abstract] Objective To investigate the transmyocardial channels changes in canines of transmyocardial jet revascularization（TMJR） filled with different hydrogel scaffolds and histocompatibility of different hydrogel scaffolds in myocardium. Methods 12 healthy mongrel canines，body weight 9～13 Kgaged 1 to 2 years old, were randomly divided into 3 groups : ①agarose hydrogel group(AH group) ②fibrin glue group(FG group) ③chitosan hydrogel group (CH group). Transmyocardial revascularization in different groups was performed with needle-free systems jeting out different hydrogel. Myocadium of three groups were obtained at 2，4，6 weeks for hematoxylin-eosin (HE) staining and Masson staining to observe the degradation and histocompatibility of the three hydrogel in myocardium and whether the channels in three groups could keep patency. Results Fibrin glue had been degraded mostly in less than 2 weeks with less inflammatory response and collagen fibers; the channels were almost occluded after the degradation of it, moreover mural thrombus were found near the channels of the endocardial surface. There were much undegraded agarose hydrogel at 6 weeks, making the collagen fibers proliferation of surrounding tissue and the channels occluded completely. The degradation cycle of chitosan hydrogel was 4 to 6 weeks and the channels could keep patency and endothelialization af