民族药铁棒锤砂炒炮制品的质量标准研究Δ-中国药房.PDF

民族药铁棒锤砂炒炮制品的质量标准研究Δ ＊ # 廖 琦 ，杨继家，张 艺，张 静（成都中医药大学民族医药学院，成都 611137） 中图分类号 R284.1；R917 文献标志码 A 文章编号 1001-0408（2013）11-1007-03 DOI 10.6039/j.issn. 1001-0408.2013.11.17 摘 要 目的：制定民族药铁棒锤砂炒炮制品的质量标准。方法：采用薄层色谱（TLC ）法对该炮制品进行定性鉴别，采用高效液 相色谱法测定样品中苯甲酰乌头原碱的含量，并对样品进行水分、灰分与浸出物测定。结果：TLC 中苯甲酰乌头原碱斑点明显，分 离度好；样品中苯甲酰乌头原碱的质量分数在0.53％～1.73％之间，水分、总灰分、酸不溶性灰分、70％乙醇浸出物的平均质量分 数分别为2.80％、2.89％、1.08％、21.38％。铁棒锤砂炒炮制品中不得检出乌头碱。结论：所建标准可用于铁棒锤砂炒炮制品的质 量控制和评价。 关键词 铁棒锤；砂炒炮制品；质量标准；薄层色谱法；高效液相色谱法；水分；灰分；浸出物 Study on Quality Standard of Stir-baked Products of Ethnic Drug Aconitum pendulum LIAO Qi ，YANG Ji-jia ，ZHANG Yi ，ZHANG Jing （College of Ethnic Medicine ，Chengdu University of TCM ， Chengdu 611137，China ） ABSTRACT OBJECTIVE ：To establish the quality standard for stir-baked products of ethnic drug Aconitum pendulum . METH- ODS ：The processed products were identified by TLC quantitatively ，and the contents of benzoylaconine in samples were deter- mined by HPLC. The determination of moisture ，ash and extracts were conducted. RESULTS ：The spots of TLC were evident and had a good degree of separation ；the mass fraction of benzoylaconine in samples were 0.53％-1.73％；average mass fraction of moisture was 2.80％，that of total ash content was 2.89％，and that of acid-insoluble ash was 1.08％. The average mass fraction of 70％ethanol extracts was 21.38％. No aconitine was found in stir-baked products of A. pendulum . CONCLUSION ：Established stan- dard is suitable for quality control and evaluation of stir-baked products of A. pendulum . KEY WORDS Aconitum pendulum ；Stir-baked products ；Quality standard ；TLC ；HPLC ；Moisture ；Ash ；Benzoylaconine 铁棒锤为毛茛科乌头属植物铁棒锤Aconitum pendulum 准的研究尚未见报道，故笔者对此进行了研究。 Busch. 和伏毛铁棒锤A. flavum Hand.-Mazz. 的干燥块根，别名 1 材料 雪上一枝蒿、三转半、黑草乌等，分布于西藏、四川西部、青海 1.1 仪器 等地区，生于海拔2800～450