三七中十八种游离氨基酸的柱前衍生化-HPLC法测定.pdfVIP

• 54 • 中间医药Z业杂志 Chinese Journal of Pharrnaceuticals 2011 , 42 (1) 王七中十八种游离氨基酸的柱前衍生化 -HPLC 法测定 黄粉11 ，玉杰l ，丁使1 ，学蜻l ，赖小平口 (1.广州中医药大学新药开发研究中心，广东广州 510006; 2. r 州中鼠药太学东莞中医药数理[程研究院， ，.尔东莞 523808) 摘要:建立了柱前衍生化幡HPLC 法同时测定主七中的 18 种游离氮某酸。以异硫佩限苯降为校前衍生化试剂，采用 C 18 色谱柱，以乙腊 -0.05 mol/L 乙酸纳缓冲液为流动相，梯度洗脱，枪测波长 254 nm。结果显示飞七恨中的游离氮主辛酸以 精氨酸、两氨酸和脯氨酸为主，约占 18 种游离氨基酸总量的 80%. 18 种锐毒草酸的平均l口]1民率为 96.5%- 103.4%, RSD 均小子 3% 。 关键词:三七:异硫曹氏酸3夜酷:牲前衍生化:游离氮J在酸:高效液相也谱:测定 中圈分类号: 0657.n 文献标志码:A 文激编号: 1001-8255 (201 1) 01-0054-的 Simultaneous Detennination of Eighteen Free Amino Acids in Panax notoginseng by Pre翩column Derivatization HPLC l l l 2 HUANG Song .2, WANG Jie , DING Jie\ LI Jing\ LAI Xiaoping • (1. Dept. ofNew Drug RD, Guαngzhou University ofChinese Medicìne. Guangzhou 510006; 2. Dongguan Mathematical Engineering Academy ofChinese Medicine, Guangzhou University ofChinese Medicine. Dongguan 523808) ABSTRACT: A pre-column derivatization HPLC method w幽 established for the determination of 18 free amino acids in Panax notoginsens. Phenylisothiocyanate was used as a derivatizing agent. A CI8 column was used with the mobile phase of acetonitrile-O.05 mol/L sodium acetate bu何泣，创 the detection wavelength of 254 nm. The results showed that the m碍。r components were arginine , alanine and proline , accounting for approximate 80% of free amino acids. The average recoveries were 96 .5 %-1 03 .4%, with RSDs less than 3 % . Key Words: Panax notoginseng; phenylisothiocyanate; pre-column derivatìzatìon; free amìno acìd; HPLC; determination 三七为五加科植物五七Pana