花生油生物精炼法去除黄曲霉毒素的研究.pdfVIP

6 CHINA OI臼 AND FATS 2012 Vol37 No. 1 油脂加工 花生油生物精炼法去除黄曲霉毒素的研究 曾凡正1 ，高 阳2 ，李进伟1 ，金青哲l ，王兴国1 (1.江南大学食品学院，江苏无锡 214122; 2. 内蒙古建筑职业技术学院，呼和浩特 01∞70) 摘要:目前已经筛选出能够固态发酵花生柏降解其中黄曲霉毒素 BJ (AFB.) 的微生物菌株，因此可 以利用发酵液提取粗酶来降解花生油中的 AFB. ，并将这种酶反应引入到花生油的精炼工艺中。通 过对比精炼工艺，将酶法脱肢(采用磷脂酶 A. )与去毒反应相结合。对脱肢去毒过程中反应温度、 NaOH 添加量、去毒粗酶添加量、磷脂酶A. 添加量、反应时间等进行单因素实验，并通过正支实验确 定脱肢去毒实验优化工艺条件为:反应温度40 C ，反应时间 4 h ， NaOH 添加量0.0259岛，磷脂酶 A. 添加量ωo mglkg。以添加 AFBJ 为 100μgI怜的花生毛油为实验对象，取样 10 g ，在去毒粗酶添加 量 l ∞ μL 及优化条件下，AFBJ 去除率高达81% ，去毒反应后花生油的脱肢效果也达到精炜、要求. (磷含量降至 13 mglkg) 。 关键词:AFB.; 生物去毒;磷脂酶 A ; 花生油;脱肢 J 申图分类号:TS224.6;TS201.6 文献标志码:A 文章编号:1003 -7969(2012)01 -α)()6 -05 Removal of af1atoxin B} in peanut oil by bio - refining ZENG Fanzheng 1 , GAO Yang2 , LI linwei 1 , JIN Qingzhe , WANG Xingguo (1. School of Food Science Technology ,Jiangnan University ,Wuxi 214122 ,Jiangsu ,China; 2. Inner Mongolia Technical College of Construction ,Hohhot 01∞70 ， China) Abstract: A bacterial strain ,which could degrade AFBJ in peanut meal by solid - state fermentation , had been screened. The crude enzyme could be extracted from the fermentation liquid produced by the bacte- rial strain to remove AFB. in peanut oil. Through compared with conditions of each refining process ,the combination of enzyme - detoxification and enzyme - degumming w描 found to be the best. Fíve factors of the combination process , such as reaction temperature , NaOH dosage , dosage of detoxification en勾m筒， dosage of phospholipase AJ ,reaction time were studied. The optimum conditions were determined 副 fol­ lows: temperature 40 C ,NaOH dosage 0.025% ,phospholipase AJ do