酵母细胞FD11b生物不对称公式(R)-(-)-扁桃酸的动力学研究.pdfVIP

Chinese J. Chem Eng. , 14(1) 73-80 (2∞6) Kinetics of Asymmetric Reduction of Phenylglyoxylic Acid to R刊 ).Mandelic Acid by Sacch现romyces Cerevisiae FDl1b XIAOMei伽n( 肖类和气 HUANG Yayan(贫雅#串户 MENG仙un(1:*) and GUO Yanghao(郭养和叫 自 Institute of Pharmaceutical Biotechnology and Enginccring, Fuzhou University, Fuzhou 35阪附1 ， αùna b Institu阻 of Ph町maceutica1险1♂neering， Huaqíao Universi凯Quanzhou 362021, China Abstract 币10 胁时酬。fa凶ymmetric production of R-(~)咽朋delic acid (R-MA) 加m phenylglyoxy Iìc acid (PGA) cata1yzed by Saccharomyces cerevÍ!nae sp. strain FDl1b was sm由时by fed-batch cultures.ηle concentrations of glucose and PGA were 00附OlI.d resp叫vely with a dual feeding syste肌When 阳electron donor 以ucose was suppHed 刷刷 I m阳 of O.0833mmolιgdw- 旷I 由e specific production rate (qp) and the enantiom叫C 以.cess of 见MAreaehed 也，emaxl­ rnum O.353mmol.gdw-1hand 97.1%, resp皿tively.ηle app阻四t reduetion activity of yeast FD 11b was obviously af- 1 fected by bo白 substrate PGA aod prod皿t MA. The q, va1ue reached fhe maxirnum 0.3←ω0.3 8mmol.gdw气h- when the PGA concentration w嗣 controlled between 25 and 35mmol-L… 1. The obvious substrate i世由ition of bioconversion 1 W础 observed at 伽e PGA concentrations higher 由.an 40mmo).L- , The accumulation of product MA also caused a severe feed-back inhibition for its production when the product concentration was above 60mmol， L飞刊e kinetic model with 由e înhibition effect ()f bo仙 substrate and product was simulated by a computer-based least-square 盯ithmatic. The eslab- lished kinetic model w出 in go