灵芝多糖glp 的抗疱疹病毒作用机理.pdfVIP

灵芝多糖 GL P 的抗疱疹病毒作用机理 1 1 1 1 1 1 1 ,2 1 刘 　静 ,阳　帆 ,李珊珊 ,王玉华 ,杨小骏 ,吴正辉 ,郜金荣 ,叶林柏 ( 1. 武汉大学生命科学学院 病毒学国家重点实验室 ,湖北 武汉 430072 ;2 . 武汉科技大学中南分校 ,湖北 武汉 430223) Inhibition of Herpes Simplex Virus Inf ection by a GL P Isolated f rom Mycel ium of Ga n oder m a l uci dum L IU J ing1 , YAN G Fan1 , L I Shanshan1 , WAN G Yuhua1 , YAN G XiaoJ un1 ,WU Zhenghui1 , GA O J inron g1 ,2 , YE Linbai1 ( 1. Col leg e of L if e S ciences , s t ate Key L aboratory of Vi rology , Wuhan Uni vers ity , Wuhan 430072 , Chi na; 2 . Z hong nan B ranch , ) Wuhan Uni vers ity of S cience an d Technol ogy , H ubei 430223 , Chi na Abstract : We rep ort ed here t hat a Gano der ma lucidum poly saccharide ( GL P) , one of t he co mpo nent s ext ract ed and p urified f ro m t he liquid fer ment atio n of mycelium of Gano derm a l uci d um , wa s active again st H SV1inf ection in V ero cell s. The CC50 (50 % cytoto xic co ncent ration) value of μ ( ) GL P for V ero cell s growt h wa s more t han 2000 g/ mL The EC50 50 % eff ective co ncent ration of μ μ GL P for vir u s yiel d reduction a ssay wa s 4 . 6 g/ mL and 11 g/ mL w hen vir u s or Vero cell s were μ p remixed wit h GL P , 17 g/ mL w hen viru s and GL P were added into t he cell cult ure simult ane μ ou sly , and 50 g/ mL w hen GL P wa s added