·药物分析与检验·.PDFVIP

·药物分析与检验· 提高坐珠达西质量标准的研究 马肖1 2* 2 2 2 ，王兰霞 ，赵建邦 ，杨锡 ，刘柏龙 (1.甘肃中医学院，兰州 730000 ；2.甘肃省食品药检所，兰州 730000) 摘要：目的 提高坐珠达西品种的质量标准。方法 采用薄层色谱法对熊胆、牛黄、木香、丁香等主要药味进行了定性 鉴别以及马钱子的液相鉴别，以高效液相色谱法对其主药西红花中西红花苷I 进行含量测定。结果 定性鉴别分离度好， 专属性强；西红花苷I 在0.031 62 ～0.284 58 μg 范围内呈良好的线形关系，r=0.999 7(n=7) ，平均回收率为 97.7% ，RSD 为 1.55%(n=6) 。结论 本法可靠，准确，专属性强，可有效控制坐珠达西的质量。 关键词：坐珠达西；薄层色谱法；高效液相色谱法；西红花苷Ⅰ；质量标准 中图分类号：R284.1 文献标志码：A 文章编号：1007-7693(2012)03-0250-04 Study on Enhancing the Quality Standard of Zuozhudaxi 1 2* 2 2 2 MA Xiao , WANG Lanxia , ZHAO Jianbang , YANG Xi , LIU Bolong (1.Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China; 2.Food and Drug Inspection Office in Gansu Province, Lanzhou 730000, China) ABSTRACT: OBJECTIVE To enhance the quality standard of Zuozhudaxi. METHODS Thin-layer chromatography (TLC) was employed to identify Bear Gall,Calculus Bovis,Aucklandia lappa Decne, Syzygium aromaticum(L.) Merr.et Perry (Clove) and high performance liquid chromatography (HPLC) was applied for the identification of Semen strychni,while high performance liquid chromatography (HPLC) was applied for the determination of Crocin- I in Croci Stigma in Zuoxidazhu. RESULTS The identification by TLC and HPLC were distinct and highly specific. A linearity was obtained from 0.031 62 μg to 0.284 58 μg of Crocin-I in Zuozhudaxi with a good correlation (r=0.9997, n=6).The average recovery was 97.7%,and RSD was 1.55(n=7). CONCLUSION The method is reliable, accurate and specific and can be used for quality control of Zuozhudaxi. KEY WORDS: Zuozhudaxi; TLC; HPLC; Crocin I; quality standard 坐珠达西收载于《卫生部药品标准中药成方 电子天平(瑞士) ；Mettler AE 240 电子天平(瑞士) ； [1] ，由寒水石、石灰华、天竺黄、 制剂藏药第一册》 KQ-250B 型超声