plant molecular biology - 生物探索-探索生物科技 .pptVIP

Ti-Plasmid Triparential mating Agrobacterium-mediated plant transformation * 根癌农杆菌介导烟草 GUS基因转化 Agrobacterium –mediated GUS gene transformation of tobacco 根癌农杆菌(Agrobacterium tumefaciens)介导转化法 A. tumefaciens is a gram-negative soil bacterium which naturally transforms plant cells, resulting in crown gall (cancer) tumors. Tumor formation is the result of the transfer, integration and expression of genes on a specific segment of tumefaciens plasmid DNA called the T-DNA (transferred DNA). The T-DNA resides on a large plasmid called Ti (tumor inducing) found in A. tumefaciens. Ti质