副猪5型 OMP分析 1-s2.0-S0264410X09009190-main.pdfVIP

Vaccine 27 (2009) 5271–5277 Contents lists available at ScienceDirect Vaccine j o u rn al h omepage: w w w. elsev /locate/vaccine Identification and characterization of novel immunogenic outer membrane proteins of Haemophilus parasuis serovar 5 Mingguang Zhou b,1, Yi Guo b,1, Jianping Zhao b , Qiaoyun Hu b , Yong Hu b , Anding Zhang a,b , Huanchun Chen a,b , Meilin Jin a,b,∗ a Unit of Animal Infectious Diseases, National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei 430070, PR China b College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei 430070, PR China a r t i c l e i n f o a b s t r a c t Article history: Haemophilus parasuis is the aetiological agent of Glässer’s disease, which is responsible for cases of Received 12 May 2009 fibrinous polyserositis, polyarthritis and meningitis in young pigs. To develop more effective vaccines, Received in revised form 11 June 2009 an immunoproteome-based approach was used to analyze the outer membrane proteins of H. parasuis Accepted 11 June 2009 serovar 5. A total of 15 proteins with high immunogenicity were identified and all were showed to be Available online 1 July 2009 immunogens for the first time in H. parasuis. Further analyses of 8 selected proteins revealed that (1) significantly higher level of serum antibodies against 6 proteins was detect