关于Arp23概要1.pptVIP

Actin polymerization is induced by Arp2/3 protein complex at the surface of Listeria monocytogenes Matthew D. Welch*, Akihiro Iwamastu Timothy J. Mitchison* Abstract The propulsion of moving of Listeria monocytogenes: actin polymerization. Which requires: bacterial surface protein ActA protein in host cell(e.g. Arp2/3 complex) Found: 8 polypeptides complex, including polypeptides fromArp2Arp3 Ref 8. Arps A family of proteins has been discovered over the past three years whose members have clear sequence homology to actin but are distinguished from actin by their structural and functional diversity. The ranks of this family, whose members are known as the actin-related proteins (arps), are expanding rapidly. Arps are but one branch of a larger superfamily which includes the actins, hsp/hsc70s, sugar kinases and several cell cycle proteins from bacteria. The existence of the superfamily has been inferred from tertiary structural data. In the case of the arps, their identification and classification has been based upon primary structural data. Placing the arps in a functional context is proving a slower process, although genetic and biochemical analyses are converging in several cases. In the past year, different arps have been linked to functions mediated by actin filaments (arp2 and arp3), microtubules (arp1) and the structural elements of chromatin (arp4 and arp6). Methods for figure 12 CYTOSKELETON: Plasma from blood bank → centrifugation washing resuspend → +lysis buffer → centrifugation ( Triton- insoluble cytoskeleton remained) →storage use CYTOPLASMIC EXTRACT: Plasma from blood bank → centrifugation washing resuspend → lyse by sonicating → centrifugation → storage use Rhodamine: 用于对线粒体染色。由于细胞内ATP的量与Rh123的荧光强度之间有相关性，因此Rh123被应用于检测细胞内的ATP。 DAPI: 是一种能够与DNA强力结合的荧光染料，常用于荧光显微镜观测。因为DAPI可以透过完整的细胞膜，它可以用于活细胞和固定细胞的染色。 Figure 1 Table 1 Figure 2 Quantification of Coomassie-dye binding: roughly equal stoichiometry SDS: molecular masses respectively → summariz