几种鸡新城疫油佐剂灭活疫苗免疫效果的比较.pdfVIP

浙江农业学报Acta Agriculturae Zhejiangen~is 19(6)：418～422，2007 几种鸡新城疫油佐剂灭活疫苗免疫效果的比较 梁华丽 ，，华炯钢 ，徐 辉2，顾亚仙 ，何永强。 ( 浙江省农业科学院病毒学与生物技术研究所，浙江杭州310021； 浙江省畜牧兽医局，浙江杭州310029， 浙江出入境检验检疫局，浙 江 杭州310012) 摘 要：比较研究了鸡新城疫(ND)Z．价灭活苗、ND灭活苗、进口ND灭活苗、ND强毒(cNDV)灭活苗和ND克 隆一30弱毒苗的免疫效果。结果表明，不同ND疫苗首次免疫鸡所产生的抗体有差异，ND二价灭活苗免疫 鸡的抗体上升最快，免疫后20 d左右可达到最高峰。1日龄免疫，14日龄攻毒，ND二价灭活苗、ND灭活苗的 保护率分别为5／5，4／5；10日龄和10日龄以上免疫，免疫后7 d或7 d以上攻毒，ND二价灭活苗、ND灭活苗、 进口ND苗的保护率分别为4／5～5／5，2／5～5／5，0／5～2／5。攻毒后ND二价灭活苗、进口ND灭活苗免疫的与 对照鸡的肛棉拭子NDV 513l生数分别为0／5，5／5，5／5。结果表明，ND二价灭活苗免疫效果优于进口ND灭活 苗、ND强毒灭活苗，稍优于ND灭活苗。 关键词：鸡新城疫灭活疫苗；新城疫抗体；攻毒试验；免疫作用 中图分类号：$852．65 文献标识码：A 文章编号：11304—1524(2007)06—0418—05 谛 Comparison on immunization effect ofinactivating of several newcastle disease oil-ad- juvant vaccines G Hua—li ，HUA Jiong—gang ，XU Hui2， GU Ya—xian ，HE Yong—qiang3 ( Virology and Biotechnology Research Insthute of Zhefiartg Academy ofAgricultural Sciences，Hangzhou 310021，Chi一 ， ； Zhejiang Provinciul Bureau ofAnimal HusbandryVeterinary Medicine，Hangzhou 310020； Zhejiang Entry-exh In— spection and Quarantine Bureau ofP．R．C，Hangzhou 310012， ) Abstract：The test compared immunization effects of five ND vaccines．The results showed that different antibody leve|s were observed in the chickens first vaceinated by different ND vaccines．The upswing of the ND antibody titer in the chickens vaccinated by the ND bivalent vaccine was fastest．The ND antibody titer attained the peak 20 d after vaccination．For the chickens vaccinated with the ND bivalent vaccine and the inactivated ND vaccine，the protection rates of vaccinated at 1． day age and attacked with virulent NDV at 14一day age were 5／5 and4／5，respectively，whereas for the chickens vaccinated th the ND bivalent inactivated vaccine，the inactivated ND vaccine and the imported inact