细辛精油对7种玉米病害的离体抑菌效果.pdfVIP

沈阳农业大学学报，2007—12，38(6)：807-810 Journal of Shenyang Agricultural University，2007—1 2，38(6)：807_8l0 细辛精油对7种玉米病害的离体抑茵效果 王桂清 ， (1．中国科学院 沈阳应用生态研究所，沈阳 110016；2．聊城大学 农学院，山东 聊城 252059) 摘要：采用生长速率法和孢子萌发法测定了细辛精油对引起玉米病害的7种病原菌(小斑病菌、弯孢霉叶斑病菌、链格孢叶斑病病 菌、圆斑病菌、顶腐病菌、青枯病菌和瘤黑粉病菌)的菌丝生长和孢子萌发的影响。结果表明，对瘤黑粉病菌、顶腐病菌、青枯病菌和 弯孢霉叶斑病病菌的菌丝生长和孢子萌发抑制效果均较好，EC 均低于700mg-L一；对小斑病菌和链格孢叶斑病病菌的孢子萌发 抑制效果较好．而对其菌丝生长的抑制效果较差；对圆斑病菌的菌丝生长和孢子萌发的抑制效果均较差，EC 均高于900mg‘L～。 对于同一种病原菌来说，细辛精油对其孢子萌发的抑制效果好于对其菌丝生长的抑制效果。 关键词：细辛；精油；玉米病害；抑菌活性 中图分类号：$435．131．4；$482．2．92 文献标识码：A 文章编号：1000—1700(2007)06-0807-04 The Antibacterial Activities of Asarum Essential Oils on Diseases in Maize in Vitro WANG Gui—qing ， f1．Institute of Applied Ecology，Chinese Academy of Sciences，Shenyang 1 10016，China； 2．College of Agronomy，Liaoeheng University，Liaocheng Shandong 252059，China) Abstract：The effects of asamm essential oils on hyphae growth and spore germination of seven pathogens (Bipolaris maydis， Curvularia lunata，Alternaria tenuis，Bipolaris carbonum，Fusarium moniliforme，Fusarium spp．，Ustsilago maydis)in maize were studied in this article．The growth rate method and spore germination method were used to determ ine the activities．The results showed that the asarum essential oils had significant antibaeterial activities on tested pathogens，while effects on spore germ ina— tion were higher than that on hyphae growth．The effects on hyphae growth and spore germination of Ustsilago maydis，Fusarium moniliforme，Fusarium spp．and Curvularia lunata were better，EC9o700mg·L～．The effects on spore germ ination of Bipolaris maydis and Alternaria tenuis were better than that on hyphae growth．The effects on hyphae growth and spore germination of Bipolaris carbonum were not better，EC90700mg·L～． Key words：asarum；essential oils；diseases in