翅果油树休眠芽和种子的试管萌发-天津中草药杂志社.PDF

　 Chinese Traditional and Herbal Drugs　 2001 32 12 · 1113· ·· 尹大泽,王小国,陈　惠 ( , 　041004) 　: 　。 　 ( 10, 11, 12, 1,2,3,), MS, 6- BA、 NAA、 GA 。　 1. 3 。 3,。2. ,: , ,; ;,、;, 70% , 。 　3。 : ;;; : R282. 21　　　: B　　　: 0253 2670(2001) 12 1113 03 Studies on germination of dormant bud and seed ofE laeagnus mollis under tisssue culturing - , - , YIN Da ze W ANG iao guo CHEN Hui 　　( Department of Biology, Shanxi NormalUniversity, Linfen Shanxi 041004, China) : 　 Abstract Object To study the factors causing the dormancy of seed and to broaden the scope in the choice of explant for thetissue cultureof the rare and near extinct plant E laeag nus moll is Diels Methods. 　 - , Dormant buds from one year old branch collected each month from Autumn to next Spring and seed of the 6- , 3 .　 　 very year were selected and cultured on basic media with the addition of BA N AAand GA Results The germination rate of dormant bud follows the regular order of the months when it was taken Those. taken in Autumn declined every month until next Spring and the nrevived monthly with those taken in, March showing the most prosperous germination The dormant bud can germinate into shoots When seed. . was cultured there are several factors leading to its dormancy hardness of the mesocarp keratinization of, : , periderm refractory to water permeation and the presence of certain inhibitory subtances Seed kernal is, . rich in nutrition but susceptible to bacterial infection leading