pdf 70855 k - 现代食品科技.pdf

现代食品科技 Modern Food Science and Technology 2016, Vol.32, No.2 Ag 沉积N、S 掺杂改性P25 材料对大肠杆菌的 抑菌性研究 王佳，张静，宋贤良，李莹影，叶盛英 （华南农业大学食品学院，广东广州510642 ） 摘要：为扩展纳米TiO 光谱响应范围，提高其可见光催化抗菌性能，本文以商业纳米TiO 为原料，采用Ag 化学沉积，N、S 2 2 非金属元素掺杂制备改性P25 抑菌材料（Ag-P25 、Ag-N-P25、Ag-S-P25 ），研究这三种材料在可见光下对大肠杆菌的抑菌效果。研究 表明，三种抑菌材料对大肠杆菌均有良好的抑制效果，其抑菌率随光照时间延长而不断上升。三种材料抑菌效果的强弱顺序为： Ag-S-P25 、Ag-N -P25、Ag-P25 。当光照时间为100 min 时，三者对大肠杆菌的抑菌率均达100%，与纯P25 的抑菌率（79% ）相对比， 提高了21%。通过扫描电镜观察和提取菌体质粒DNA 的电泳检测表明，三种材料对大肠杆菌菌体造成破坏，引起DNA 泄漏，导致 菌体死亡。通过研究讨论改性P25 材料对大肠杆菌的灭活机理，可为具有抗菌功能的新型保鲜材料的研究开发提供理论和实验依据。 关键词：P25 TiO 纳米颗粒；掺杂；大肠杆菌；抑菌性 2 文章篇号：1673-9078(2016)2-79-83 DOI: 10.13982/j.mfst.1673-9078.2016.2.013 Study on the Antibacterial Property of Ag-deposited N-/S-doped TiO2 P25 Nanoparticles against Escherichia coli WANG Jia, ZHANG Jing, SONG Xian-liang, LI Ying-ying, YE Sheng-ying (College of Food Science，South China Agricultural University, Guangzhou 510642, China) Abstract: To broaden the spectral response of nanoscale TiO2 and to enhance its visible-light photocatalytic antibacterial activity, in this study, chemical deposition of silver (Ag) and the doping of nonmetal elements nitrogen (N) and sulfur (S) was performed to prepare modified P25 antibacterial materials (Ag-P25, Ag-N-P25, and Ag-S-P25) with commercial TiO2 nanoparticles as the raw material. The antibacterial effects of these three P25 materials were studied against Escherichia coli under visible light. The study showed that all the three antibacterial materials had good inhibitory effects on E. coli g