管理课件细胞色素c能诱导植物细胞编程性死亡.pdfVIP

植 　物 　学 　报 　1999 , 41 (4) :379～383 Acta Botanica Sinica 细胞色素 c 能诱导植物细胞编程性死亡 孙英丽 　赵 　允 　刘春香 　翟中和 (北京大学生命科学学院　北京 　100871) ( ) ( ) 摘要 　以悬浮培养的胡萝 卜 Daucus carota L . 与烟草 Nicotiana tabacum L . cv. BY2 细胞原生质体为材料 ,加入一 定浓度的细胞色素 c 和 dATP 。不同取样时间的DAPI 荧光染色与电镜超薄切片观察的结果显示染色质发生凝集 、 ( ) 趋边化 ,最终形成凋亡小体 。核酸电泳显示 DNA 发生特异降解并形成电泳“阶梯”DNA ladder 。用末端脱氧核糖 ( ) 核酸转移酶介导的 dUTP 切口末端标记方法 TUNEL 检测发现 DNA 的3’OH 断端被原位特异标记 。以上结果说明: 细胞色素 c 能诱导植物细胞发生典型的凋亡 。 关键词 　细胞色素 c , 编程性细胞死亡 , 细胞核 Cytochrome c Can Induce Progra mmed Cell Death in Plant Cells SUN YingLi 　ZHAO Yun 　L IU ChunXiang 　ZHAI ZhongHe ( College of L if e Sciences , Peking University , Beij ing 100871) Abstract 　The study revealed the detection of stereotypic hallmarks of the apoptosis during cell death induced by cytochrome c in carrot ( Daucus carota L . ) and tobacco ( N icotiana tabacum L . ) protoplasts. The progres sive delineation of fragmented DNA into distinct bodies , coincident with the appearance of DNA ladders , was also observed during death of the cytochrome ctreated carrot and tobacco protoplasts. TUNEL analysis of cells revealed DNA fragmentation localized in the dying cells as well as the additional formation of apoptoticlike bodies. These results suggest that cytochrome c can induce programmed cell death (PCD) in plant cells ; also indicate that PCD in plants involves some of the characteris