慢性应激对大鼠免疫功能损伤机制的实验研究.pdfVIP

环境与职业医学2007年l2月第24卷第6期 J Environ Occup Med，Dec．2007 Vo1．24 No．6 · 599 · 文章编号：1006．3617(2007)06—0599—04 中图分类号：R363．1 文献标识码 ：A 【论著】 慢性应激对大鼠免疫功能损伤机制的实验研究 刘继文，袁芳，连玉龙，杨晓燕 摘要： [目的]研究慢性应激对大鼠免疫功能的影响及其作用机制。 [方法]通过建立慢性应激动物模型，免 疫组织化学法检测脾脏T淋巴细胞亚群的阳性率，电镜观察脾脏超微结构的改变，黄嘌呤氧化酶法测定超氧化物歧化 酶(SOD)活性，硫代巴比妥酸(TBA)法测定丙二醇(MDA)含量，彗星实验评价大鼠外周血淋巴细胞DNA损伤情况。[结 果]与对照组相比，实验组大鼠脾脏超徽结构异常明显，T淋巴细胞亚群比例异常，CD3 T细胞百分率、CD4+／CD8 值均 下降(P0．05)，CD8 T细胞百分率升高(P0．01)，血清SOD活性(56．70±15．65)U／ml降低，MDA含量(9．21± 2．75)nmol／ml升高(P0．05)，彗星细胞发生率升高(P0．05 o淋巴细胞DNA损伤与MDA含量呈正相关，SOD活性与 CD3 T细胞、CD4 ／CD8 比值呈正相关；MDA含量与细胞免疫功能下降水平呈正相关，淋巴细胞DNA损伤与细胞免疫功 能下降水平呈正相关。 [结论]慢性应激使大鼠血清MDA含量增加，SOD活性下降，脾脏超微结构改变，损伤外周血 淋巴细胞DNA，从而影响机体的免疫功能。 关键词：应激；免疫功能；脂质过氧化；DNA损伤；大鼠 Experimental Study on the Mechanism of Chronic Stress on Immune Function Damage 0f Rat上 -，j— wen，YUANFang，LIANYu—long，YANGXiao-yan(DepartmentofOccupationalandEnvironmentalHealth， XinjiangMedical University，Urumqi 830011，C ina) Abstract： [Objective]To study the mechanism of immune function damage of rat under chronic stress．[Methods] T cell subpopulations was measured by the method of immunohisto-chemistry in chronic stress model rats and in control group．the ultrastructural changes ofits spleen were also observed．The activity ofsertlm superoxide dismutase(SOD)was measured with xanthine oxidase method and the contents of malonaldehyde(MDA)with thiobarbitttric acid(TBA)．The degree of DNA damage in peripheral blood lymphocytes was analyzed with comet assay．1 Results]Some pathomorphism changes in spleen were observed in stress model rats．The percent of lymphocyte appeared some alterations，CD3+]r cell numbers and the ratio of CD4+／CD8 were decreased(P0．05)， but CD8+T cell were increasedin test group rats(P0．01)．The activities ofSOD werelower(56．70 4-15．65)U ，