用于药物筛选的微流控细胞阵列芯片.PDF

生 物 工 程 学 报 Chin J Biotech 2009, May 25; 25(5): 779-785 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn © 2009 Institute of Microbiology, CAS CSM, All rights reserved 生物技术与方法 1,2 1,2 1,2 1 1 郑允焕 , 吴建璋 , 邵建波 , 金庆辉 , 赵建龙 1 , 200050 2 , 100039 : 细胞区域分布培养以及如何有效地对微流体进行操控是微流控阵列芯片在细胞药物研究中的关键技术。本研 究介绍了一种利用SU-8 负性光刻胶模具和PDMS 制作双层结构的微流控细胞阵列芯片的方法, 该芯片通过C 型的坝结 构将进样细胞拦截在芯片的细胞培养的固定区域, 键合双层 PDMS 构成阀控制层, 阀网络的开关作用成功实现了芯片 通道内微流体的操控, 同时芯片设计了药物浓度梯度网络, 产生6 个不同浓度的药物刺激细胞。通过对芯片3 种共培养 细胞活性的检测和药物伊立替康(CTP-11)对肝癌细胞的浓度梯度刺激等实验结果验证该芯片在细胞研究和药物筛选等 方面的可行性。 : 微流控芯片, 药物筛选, C 型坝结构, 微阀, 药物浓度梯度, 细胞共培养 Microfluidic cell culture array chip for drug screening assays 1,2 1,2 1,2 1 1 Yunhuan Zheng , Jianzhang Wu , Jianbo Shao , Qinghui Jin , and Jianlong Zhao 1 Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China 2 Graduate School of Chinese Academy of Sciences, Beijing 100039, China Abstract: We developed a novel microfluidic cell chip, which enabled drug delivery, fluid control and cell co-culture. The device consisted of an array of 6×6 cell culture chambers, a drug gradient generator and fluidic control valves. Micro-dam structures of the chambers were able to trap cells while loading and drug gradient network generated drug gradient of 6 different concentrations. Also we applied hydraulic valves to control the microfluid and simu