您所在位置网站首页 > 海量文档  > 教育文化 > 教育文化

Hormonal induction of ovulation and spermiation in Atlantic cod - 副本.doc 8页

本文档一共被下载: ,您可全文免费在线阅读后下载本文档。

  • 支付并下载
  • 收藏该文档
  • 百度一下本文档
  • 修改文档简介


Hormonal induction of ovulation and spermiation in Atlantic cod (Gadus morhua) Captive, wild-caught female Atlantic cod, Gadus morhua, from Georges Bank (n=19) were divided into three treatment groups based on body weight and diameter of the largest clutch of oocytes present. Treatments administered were an intramuscular implant of one of two gonadotropin releasing hormone analogues ? (DAla6, Pro9-NHEt)-LHRH ethylamide (LHRHa, 150 μg) and salmon gonadotropin releasing hormone analogue (sGnRHa, 150 μg, OvaplantR), or a saline injection (control). Over a period of 20 days, 12 of 14 implanted and one control fish ovulated. There was no significant variation between egg volumes and percent fertilization (P≥0.4968) of implant-treated and naturally ovulating females; however, significant variation did exist for the estimated number of hatched larvae among treatments (P=0.0169). Non-spermiating captive wildcaught Atlantic cod males (n=11 Georges Bank) were divided into two treatment groups (OvaplantR and saline control) based on body weight. Large volumes of milt with high motility and spermatocrits were obtained from each of five OvaplantR-treated males starting 6 days post-implant. Over a 27 day period, each of six control males produced milt at least once, but in small volumes with variable motility and low spermatocrits. Milt volumes of the OvaplantR-treated males were higher than naturally spermiating males (P=0.0002), but had similar motilities and spermatocrits (P≥0.0462). These results demonstrate the effectiveness of GnRHa implants in inducing ovulation and spermiation in Atlantic cod broodstock without adversely affecting gamete quality. 1. Introduction A general decline in Atlantic cod (Gadus morhua) stocks and continuous, high consumer demand has stimulated the development of cod aquaculture in several countries, including Canada, Iceland, Ireland, Norway and the United States (Rosenlund and Skretting, 2006). The development of this industry has benefited from


用户名: 验证码: 点击我更换图片

“原创力文档”前称为“文档投稿赚钱网”,本站为“文档C2C交易模式”,即用户上传的文档直接卖给(下载)用户,本站只是中间服务平台,本站所有文档下载所得的收益归上传人(含作者)所有【成交的100%(原创)】。原创力文档是网络服务平台方,若您的权利被侵害,侵权客服QQ:3005833200 电话:19940600175 欢迎举报,上传者QQ群:784321556