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Hormonal induction of ovulation and spermiation in Atlantic cod - 副本
Hormonal induction of ovulation and spermiation in Atlantic cod (Gadus morhua)
Captive, wild-caught female Atlantic cod, Gadus morhua, from Georges Bank (n=19) were divided into three
treatment groups based on body weight and diameter of the largest clutch of oocytes present. Treatments
administered were an intramuscular implant of one of two gonadotropin releasing hormone analogues ? (DAla6,
Pro9-NHEt)-LHRH ethylamide (LHRHa, 150 μg) and salmon gonadotropin releasing hormone analogue
(sGnRHa, 150 μg, OvaplantR), or a saline injection (control). Over a period of 20 days, 12 of 14 implanted and
one control fish ovulated. There was no significant variation between egg volumes and percent fertilization
(P≥0.4968) of implant-treated and naturally ovulating females; however, significant variation did exist for
the estimated number of hatched larvae among treatments (P=0.0169). Non-spermiating captive wildcaught
Atlantic cod males (n=11 Georges Bank) were divided into two treatment groups (OvaplantR and
saline control) based on body weight. Large volumes of milt with high motility and spermatocrits were
obtained from each of five OvaplantR-treated males starting 6 days post-implant. Over a 27 day period, each
of six control males produced milt at least once, but in small volumes with variable motility and low
spermatocrits. Milt volumes of the OvaplantR-treated males were higher than naturally spermiating males
(P=0.0002), but had similar motilities and spermatocrits (P≥0.0462). These results demonstrate the
effectiveness of GnRHa implants in inducing ovulation and spermiation in Atlantic cod broodstock without
adversely affecting gamete quality.
1. Introduction
A general decline in Atlantic cod (Gadus morhua) stocks and
continuous, high consumer demand has stimulated the development
of cod aquaculture in several countries, including Canada, Iceland,
Ireland, Norway and the United States (Rosenlund and Skretting,
2006). The development of this industry has benefited from
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