神经病理性痛大鼠背根神经节细胞电生理学特征的改变3.pdf

中国疼痛医学杂志 Chinese Journal of Pain Medicine 2003 ,9 , ( 1) ·31 · 神经病理性痛大鼠背根神经节 细胞电生理学特征的改变 邓伦斌 　姚 　磊 　王贺春 　曹晓杰 　万 　有 　韩济生 (北京大学神经科学研究所、神经生物学系、教育部神经科学重点实验室、脑科学与认知科学中心 , 北京 100083) ( ) 摘要 　目的:研究神经病理性痛大鼠背根神经节 dorsal root ganglion , DR G 细胞电生理特征的改 变。方 法 : 以单侧L 5 和 L 6 脊神经结扎制备大鼠神经病理性痛模型 ,运用全细胞电流钳技术进行 电生理记录。结果:引起损伤侧 D GR 中、小直径神经元兴奋的基强度降低 ,动作电位爆发阈值下 降 ,小直径神经元的动作电位时程变宽 , 自发放电的细胞比率明显升高。结论:神经损伤诱导初级 感觉神经元的兴奋性增强 ,这可能是引起动物神经病理性痛敏的原因之一。 关键词 　神经病理性痛 ;动作电位 ;背根神经节 CHANGES OF EL ECTROPHYSIOLOGICAL PROPERTIES IN DORSAL ROOT GANGL ION NEU RONS LSOLATED FROM NEUROPATHIC PAIN RATS DEN G LunBin , YAO Lei , WAN G HeChun , CAO XiaoJie , WAN You , HAN JiSheng (Neuroscience Research Institute , Peking University , 38 Xueyuan Road , Beijin g 100083) Abstract 　Objective : Pain and h yperalgesia can occur when nerves were injured. The present study aims to examine the electrophysiolo gical changes of dorsal root ganglion (DR G) neurons in rats with neuropathic pain. Methods : The neuropathic pain model in rat was prepared by tight ligation of right side L5 and L6 spinal nerve. Whole cell current clamp technique was used for electrophysiolo gical recording. Results : The rheobase and the threshold of action potential were reduced in both small and medium DR G neurons after nerve ligation , while the duration of action potential at half amplitude (APD0. 5) was broadened in small injured neurons. Nerve injur y also induced spontaneous activities in small neurons. No significant changes were observed in other electrophysiolo gical properties. Conclu sion : These results suggested that nerve ligation