氯化三乙基锡对体外培养大鼠C6脑胶质瘤细胞增殖和.docVIP

　　氯化三乙基锡对体外培养大鼠C6脑胶质瘤细胞增殖和 【摘要】 目的 探讨氯化三乙基锡(TETC)对体外培养大鼠C6脑胶质瘤细胞增殖和凋亡的影响。方法 采用MTT法检测0.5，1.0和2.0 μmol/L TETC对体外培养大鼠C6脑胶质瘤细胞作用24 h和48 h后细胞增殖的影响、HE染色细胞形态学观察0.5，1.0和2.0 μmol/L TETC对体外培养大鼠C6脑胶质瘤细胞作用48 h后细胞增殖和形态学变化。采用透射电镜观察0.5，1.0和2.0 μmol/L TETC作用C6细胞48 h后的超微形态学变化。采用荧光原位末端标记方法检测细胞凋亡。结果 MTT法及HE染色细胞形态学观察均显示0.5，1.0和2.0 μmol/L TETC在体外均可抑制C6细胞增殖，C6细胞增殖率存在着剂量依赖性和时间依赖性降低趋势;HE染色和透射电镜可观察到C6细胞凋亡的形态学变化。荧光原位末端标记方法检测到细胞凋亡率存在剂量依赖性上升趋势。结论 TETC可抑制体外培养大鼠C6胶质瘤细胞增殖，其机制可能主要与C6细胞凋亡有关。 【关键词】 三乙基锡化合物;MTT法;C6脑胶质瘤;增殖;凋亡 　　【Abstract】 Objective To study the effects of triethyltin chloride (TETC) on the proliferation and apoptosis of cultured rat C6 glioma cells. Methods MTT assay ed to evaluate the effects of 0.5, 1.0 and 2.0 μmol/L TETC on the proliferation of rat C6 glioma cells for 24 and 48 h. HE staining and electron microscope ployed respectively for determining the proliferation effect and detecting the apoptosis of C6 glioma cells treated by 0.5, 1.0 and 2.0 μmol/L TETC for 48 h. Fluorescent TUNEL assay easure the percentage of apoptosis in C6 glioma cells treated by 0.5, 1.0 and 2.0 μmol/L TETC for 48 h.Results 0.5, 1.0 and 2.0 μmol/L TETC could inhibit the proliferation of cultured C6 glioma cells in vitro performed by both MTT assay and HE staining, and the proliferation rate edependent manner. Apoptotic changes a cells stained icroscope or in part of TETC treated C6 glioma cells observed by electron microscope. The apoptosis rate, measured by fluorescent TUNEL assay, anner in TETC treated C6 glioma cells. Conclusions TETC inhibits the proliferation of C6 glioma cells in vitro, the mechanism of ay be mainly involved in apoptosis of C6 glioma cells. 　　【Key a; Proliferation; Apoptosis 　　寻找易透过血脑屏障且有效的抗胶质瘤药物是亟待解决的问题。氯化三乙基锡(TETC)是中枢神经系统毒物，具有脂溶性，易透过血脑屏障。关于TETC治疗脑胶质瘤国内外尚未见报道。本实验旨在研究TETC对体外培养大鼠C6 胶质瘤细胞增殖和凋亡的影响，为治疗胶质瘤提供理论依据。 　　1 材料与方法 　　1.1 材料 氯化三乙基锡TETC(Alfar Aesar 美国)，四甲基偶氮唑盐MTT(Sigma)，二甲基亚砜DMSO(Sigma)，IMDM细胞培养基(Gibco)，胎牛血清 (中国医学科学院生物工程研究所)ApoAlertTM DNA Fragmentation检测试剂盒(BDbioscience)。 　　1