原花青素对大鼠脑缺血再灌注损伤的保护作用.pdfVIP

2008年6月第46卷第l8期 · 基础研究· 原花青素对大鼠脑缺血再灌注损伤的保护作用 李 峰 谢 海 高 颖 ’ 战同霞。 (1．山东省潍坊市人民医院保健科，山东潍坊261041；2．潍坊医学院，山东潍坊261053) 【摘要】目的 研究原花青素对大鼠脑缺血再灌注损伤脑组织神经生长因子表达的影响。方法 SD大鼠48只随机分4组： 假手术组、缺血再灌注模型组、GSP大剂量组、GSP小剂量组，每组12只，应用线栓法制作大鼠大脑中动脉缺血再灌注损伤 模型，术后观察动物神经行为学变化并进行神经病学评分，采用免疫组织化学染色检测各组半暗带区颞顶部脑皮质神经生 长因子的表达。结果 神经功能评分显示模型组有明显的神经功能缺损症状，GSP用药组神经功能评分明显低于模型组， 差异有统计学意义(P0．05)；与模型组比较，各GSP组神经生长因子的表达明显升高，差异有统计学意义(P0．05)。结论 原花青素可上调脑缺血再灌注后缺血脑组织内源性神经生长因子的表达，而起到脑保护作用。 【关键词】原花青素；脑缺血再灌注；神经生长因子 【中图分类号】R915 【文献标识码】A 【文章编号】1673—9701(2008)18-95—03 Protective Effects of Procyanidin on Cerebral Ischemia Reperfusion In- jury in Rats u Feng1 X1E H GAO Ying~ ZHAN Tongxia2 1．Department of HeMth，Weifang People’S Hospital，Shandong 261041；2．Weifang Medical College，Shandong 261053 【Abstract】0bjecfive To study the effects of grape seed procyanidin(GSP)on the expression of nerve growth factor(NGF)after cerebral ischemia reperfusion injury(CIBI)in rats．Methods 48 rats were divided randomly into four groups(n=12)：sham oper~ion group， ischemia-reperfusion(I／R)model groups，lose dose GSP(40mg／kg)group，high dose GSP(1Omg／kg)group．Reversible middle cerebral arte~occlusion(MCAO)model was established by intraluminal suture technique．Sham oper~ion group rats were operated while middle cerebral artery were not blocked．After 2h of occlusion and 48h of reperfusion，using behaviour test，evaluate the neurological deficiency of each group．The expressions of NGF in the cortex were measured by immunohistochemical method．Results The nerves function injuries were severe in model group rats，neurological score of rats treated with GSP was lower apparently compared with model group rats (P0．05)； Immunohistochemical studies showed that compared with I／R model group and the expression of NGF was up-regulated remarkedly in GSP group