白腐菌混合菌降解木质素最佳条件的优化 - 生物技术通报.pdf

生物技术通报 ·研究报告 · B IO TECHN OLO G Y　BULL E T IN 2009年第 12 期 白腐菌混合菌降解木质素最佳条件的优化 段传人 贾秋云 (重庆大学生物工程学院 ,重庆 400030) 　　摘 　要 : 　通过正交试验对 3种白腐菌混合菌降解竹材木质素的条件进行优化 ,结果表明 ,在温度为 32 ℃、pH30、固体发 酵时间 20 d、培养液与竹材基质质量百分比 110%时降解木质素的效率最高 。在此基础上 ,研究了两种诱导剂对 白腐菌混合 菌降解木质素的影响 。结果表明 ,两种诱导剂均能促进木质素的降解 ,其中 H O 在浓度 1%时 ,木质素降解率高达 62 9% ,苯 2 2 甲酸在浓度 01%时 ,木质素降解率最高 ,为 678% 。 关键词 : 　白腐菌 固体发酵 木质素降解率 诱导剂 O ptim iza tion of the Cond ition s for W h iterot FungusM ixed Bacter ia D egrad ing L ign in Duan Chuanren J ia Q iuyun (B ioeng ineering Colleg e of Chong qing Un iversity, Chongqing 400030) 　　Ab s tra c t: 　In th is p ap er, the condition s for W h iterot fungu s m ixed bacteria degrading lign in were op tim ized through the orthogo nalexp erim en ts. The re su lts showed that when the temp eratu re wa s 32 ℃, pH30, the solidstate ferm entation tim e wa s 20 d, the cu l ture m edium and the sub strate quality bam boo wa s 110% , and the efficiency of lign in degradation was up to a m axim um On th is ba sis, the imp act of two k ind s of inducing agen ts acting on m ixed wh ite rot fungi degrade lign in wa s studied The re su lts showed that two k ind s of inducing agents can p romote the degradation of lign in, in wh ich the concentration of H O at 1% , lign in degradation rate a s 2 2 h igh as 62 9% , benzo ic acid in the concen tration of 01% , and the h ighest rate of lign in degradat