贵州70份玉米自交系的SSR标记遗传多样性及其杂种优势群分析_杨文鹏.docxVIP

植物遗传资源学报2011,12(2):241-248JournalofPlantGeneticResources贵州70份玉米自交系的SSR标记遗传多样性及其杂种优势群分析杨文鹏1,2,关　琦3,杨留启1,王　伟1,张文龙4,祝云芳1,潘敏娜1,沈建华1,赵　致3(1贵州省旱粮研究所,贵阳550006;2贵州省农业科学院/贵州省农业生物技术重点实验室,贵阳550006;3贵州大学农学院,贵阳550025;4贵州省种子总站,贵阳550001)　　摘要:从251个SSR标记中筛选出均匀分布在玉米基因组上的88个SSR标记,用以分析评价贵州省2000年以来47个审定品种的70份亲本材料的遗传多样性。SSR标记检测的结果:88个标记共检测出466个等位基因,每个标记可检测等位基因2～18个,平均为5.31个;每个标记位点的多态性信息量(PIC)变化为0.213～0.965,平均为0.586,这表明贵州玉米自交系具有较为丰富的遗传多样性。POPTREE聚类分析结果:70份自交系分为Ⅰ、Ⅱ和Ⅲ类群。Ⅰ类群含8个自交系,以瑞德和兰卡斯特等温带种质为主。Ⅱ类群有11个自交系,以PN78599、瑞德和兰卡斯特等温带种质为主。Ⅲ类群拥有51个自交系,可分为A和B2个亚群,B亚群还可再分为B1和B22个次亚群,A亚群中的10个系以我国地方温带种质为主,B1次亚群中的19个系以贵州地方亚热带种质为主,B2次亚群中的22个系以泰国苏湾热带种质为主。杂种优势利用分析的结果表明,贵州近些年在玉米育种中,主要是利用贵州地方亚热带种质和泰国苏湾热带种质2个杂种优势群,这与其多态位点百分率较高有关,与其群内SSR位点的平均等位数较多有关。贵州玉米育种利用的种质类型较少,有必要加强玉米种质的扩增、改良与创新研究。关键词:自交系;SSR标记;遗传多样性;杂种优势群;玉米GeneticDiversityandHeteroticGroupof70MaizeInbredLinesinGuizhoubySSRMarker1,2,GUANQi3,YANGLiu-qi1,WANGWei1,ZHANGWen-long4,YANGWen-peng1,PANMin-na1,SHENJian-hua1,ZHAOZhi3ZHUYun-fang(1GuizhouInstituteofUplandFoodCrops,Guiyang550006;2GuizhouKeyLaboratoryofAgriculturalBiotechnology/GuizhouAcademyofAgriculturalSciences,Guiyang550006;3Agronomiccollege,GuizhouUniversity,Guiyang550025;4GuizhouGeneralSeedStation,Guiyang550001)　　Abstract:Geneticdiversityof70parentalinbredlinesof47maizehybridsauthorizedinGuizhouprovincesince2000wasanalyzedbyusing88simplerepeatsequence(SSR)markersequablydistributedingenomeandscreenedfrom251SSRmarkersthroughwholegenome.466allelesweredetectedacross88SSRloci;5.31(2-18)perlocus,averagely.Theaveragepolymorphicinformationcontent(PIC)was0.586(0.213-0.965)perlocus.Allaboveshowedthatthegeneticdiversityofinbredlinestestedwasrelativelyplentiful.The70inbredscouldbeclassi-stGroupincluded8inbredlinesmainlywith fiedintothreegroupsbyPOPTREEsoftware(Windowsversion).The1ndGrouphad11inbredsmainlywithtemperategermplasmoftemperategermplasmofReid,Lancaster,etc..The2rdGrouppossessed51lines,andcouldbesortedintoAandBsub-groupPN78599,Reid,Lancaster,etc..The3whichthelattercouldbedividedintoB1andB2sub-sub-grou