糖尿病对乳腺癌耐药蛋白表达和功能的组织特异性 - researchgate.pdf

See discussions, stats, and author profiles for this publication at: /publication/287869318 Tissue-specific damage in expression and function of breast cancer resistance protein in streptozotocin-induced diabetic rats Article in ournal of China Pharmaceutical University · December 2011 CITATIONS READS 3 20 5 authors, including: ie Li Xiao-Dong Liu Xishuangbanna Tropical Botanical Garden China Pharmaceutical University 1,126 PUBLICATIONS 8,2 5 CITATIONS 123 PUBLICATIONS 1, 38 CITATIONS SEE PROFILE SEE PROFILE Some of the authors of this publication are also working on these related projects: Evolutionary distinctiveness and conservation priority of the endangered Najas ancistrocarpa (Hydrocharitaceae) View project REDS-II View project All content following this page was uploaded by Xiao-Dong Liu on 04 une 2016. The user has requested enhancement of the downloaded file. 学 报 ５４４ ＪｏｕｒｎａｌｏｆＣｈｉｎａＰｈａｒｍａｃｅｕｔｉｃａｌＵｎｉｖｅｒｓｉｔｙ　２０１１，４２（６）：５４４－５５０ 糖尿病对乳腺癌耐药蛋白表达和功能的组织 特异性损伤作用 张璐璐，金　实，李　佳，段　茹，刘晓东 （中国药科大学药物代谢动力学重点实验室，南京２１０００９） 摘　要　首先从在体水平研究糖尿病能否导致大鼠皮层、海马、肝、肠黏膜和肾中乳腺癌耐药蛋白（ＢＣＲＰ）的功能和表 达发生改变以及胰岛素治疗能否逆转糖尿病引起的这种改变；进一步通过体外Ｃａｃｏ２和ＨｅｐＧ２细胞模型研究糖尿病血清 中是否存在异常成分影响ＢＣＲＰ的功能和表达。通过单次给予链脲菌素（６５ｍｇ／ｋｇ，ｉｐ）诱导糖尿病模型，三磷酸腺苷结合 转运蛋白Ｇ超家族成员２（ＡＢＣＧ２）／ＢＣＲＰ的表达分别由ＱＴＰＣＲ和Ｗｅｓｔｅｒｎｂｌｏｔ实验测定，体外通过测定Ｃａｃｏ２和ＨｅｐＧ２ 细胞中哌唑嗪的摄取研究ＢＣＲＰ的功能改变。在体ＱＴＰＣＲ分析结果表明：５周和８周糖尿病大鼠的皮层、肝和肠黏膜中， ＡＢＣＧ２的表达均显著降低，而在海马和肾中其表达却显著增加。Ｗｅｓｔｅｒｎｂｌｏｔ实验结果在大部分组织中与ＱＴＰＣＲ结果相 一致。离体实验结果表明在Ｃａｃｏ２和ＨｅｐＧ２细胞中，高胰岛素和