卵黄囊移植人脐血cd34+细胞构建人源化血液系统小鼠模型 .pdf

中国组织工程研究与临床康复 第12 卷 第43 期 2008 –10 –21 出版 基础医学 Journal of Clinical Rehabilitative Tissue Engineering Research October 21, 2008 Vol.12 No.430 + ★◆ 卵黄囊移植人脐血CD34 细胞构建人源化血液系统小鼠模型 1,2 2 2 1 2 陈 林 ，雍 军 ，张鹏博，吴应积，邓宏魁 + Construction of humanized blood mouse models using human cord blood CD34 cells by yolk sac transplantation 1,2 2 2 1 2 Chen Lin , Yong Jun , Zhang Peng-bo , Wu Ying-ji , Deng Hong-kui Abstract 1 Key Laboratory of Mammal Reproductive BACKGROUND: Compared with utero transplantation in large animals such as goat, the utero heterogeneous hematopoietic stem Biology and cell transplantation in small animals such as mouse is facing difficulties. Rebuilding rate of heterogeneous hematopoietic stem cells in Biotechnology, Ministry of Education, peripheral blood was below 1%. Innermonglia OBJECTIVE: To observe rebuilding rate of heterogeneous hemopoiesis following human cord blood hematopoietic stem cell University, Huhhot transplantation in mouse yolk sac, and to compare with outcomes of abdominal transplantation. 010000, Nei Monggol DESIGN, TIME AND SETTING: The cytology in vivo experiment was performed at the Laboratory of Stem Cells and Regenerative Autonomous Region, China; 2Laboratory of Biology, Beijing University in October 2007. Stem Cells and MATERIALS: Cord blood from healthy newborns was collected at the Maternal and Child Health Hospital of Beijing Haidian Regenerative Biology, District. ICR mice were obtained fr