ztc1+1-ⅱ澄清剂用于昆参片水提液的纯化研究 - 食品与药品.pdf

172 食品与药品 Food and Drug 2011年第 13卷第05期 ZTC1+1- Ⅱ澄清剂用于昆参片水提液的纯化研究 朱立俏，盛华刚 （山东中医药大学药学院，山东 济南 250355 ） 摘 要：目的 研究纯化昆参片水提液的工艺。方法 以纯化前后昆参片水提液中总多糖含量和浸膏率为考察指标，比较乙 醇沉淀法、壳聚糖澄清剂和ZTC 1+ 1- Ⅱ澄清剂对提取液的纯化效果，并用单因素考察法确定ZTC 1+ 1- Ⅱ澄清剂的最佳纯化 工艺。结果 ZTC 1+ 1- Ⅱ澄清剂优于乙醇沉淀法和壳聚糖澄清剂，最佳纯化工艺为澄清剂用量5 %B+2.5 %A ，药液浓度 0.2 g/mL ，搅拌速度100 r/min 。结论 ZTC1+ 1- Ⅱ澄清剂可用于纯化昆参片水提液。 关键词：昆参片；ZTC1+ 1- Ⅱ澄清剂；纯化 中图分类号：R979.1 文献标识码：A 文章编号：1672-979X （2011）05-0172-04 Study on Purification Process of Water Extract of Kunshen Tablet with ZTC1+1- Clarificant Ⅱ ZHU Li-qiao, SHENG Hua-gang (Pharmacy School of Shandong University of Traditional Chinese Medicine, Jinan 250355, China) Abstract: Objective To study the purification process of the water extract of Kunshen Tablet with ZTC 1+ 1- Ⅱ clarificant. Methods The purification efficacy of ethanol precipitation, chitosan clarificant and ZTC1+ 1- Ⅱclarificant was compared, using the content of total polysaccharides and dry extract rate as performance indicators. The optimum purification process was determined by single factor test. Results The purification effect of ZTC1+ 1- Ⅱclarificant was better than that of ethanol precipitation and chitosan clarificant. The optimum purification process was as follows: the concentration was 0.2g•mL-1, the amount of ZTC1+ 1- Ⅱclarificant was 5%B+2.5%A, the stirring speed was 100 r•min-1. Conclusion ZTC1+ 1- Ⅱclarificant can be used in purification process of the water extract of Kunshen Tablet. Key Words: Kunshen Tablet; ZTC1+ 1- Ⅱclarificant; purification 昆参片由昆布、人参、藤梨根等药组成，为临床 ZTC 1+ 1- Ⅱ澄清剂纯化提取液的效果，并用单因素考 使用多年的治疗进展期胃癌的验方，原方以汤剂为 察法研究了ZTC1+ 1- Ⅱ澄清剂的最佳纯化工艺。 用，疗效可靠，现研制成片剂。昆布主要含有多糖类