大肠杆菌生物膜的筛选及生长曲线测定 - 中国农学通报.pdf

中国农学通报 2014,30(5):23-27 Chinese Agricultural Science Bulletin 大肠杆菌生物膜的筛选及生长曲线测定 喻华英，李启峰 （塔里木大学动物科学学院，新疆阿拉尔843300） 摘 要：为探究犊牛腹泻病中大肠杆菌生物膜的形成过程。本试验利用96 孔微量板法，对分离鉴别的犊 牛腹泻粪便中的60 株大肠杆菌进行生物膜的筛选和统计。结果表明：得到了9 株生物膜阳性大肠杆菌， 并测定出大肠杆菌生物膜阳性菌的生长曲线。从而确定大肠杆菌生物膜形成的四个时期：初粘附、粘 附、生物膜的成熟、脱落等过程且不同大肠杆菌生物膜形成时间不尽相同。为临床上治疗犊牛腹泻病提 供了新的理论依据。 关键词：大肠杆菌；生物膜；筛选；生长曲线 中图分类号：R3782+2 Q735 文献标志码：A 论文编号：2013-1796 Screening Biofilm and Determination Growth Biofilm of Curve in Escherichia coli Yu Huaying, Li Qifeng (CollegeOfAnimalScienceTarimUniversity, Alar Xinjiang 843300) Abstract: The biofilm explored the formation process of E. coli in the calf diarrhea. The experiment used the 96-well micro plate method screened and counted biofilm in 60 strains in E. coli from calf diarrhea fecal which were separated and identified in laboratory. The results showed that get nine positive biofilms in E. coli and determined the positive biofilm growth curve in E. coli. The test concluded and known the biofilm formation period contained four stages: initial adhesion, adhesion, mature biofilm and shedding processes, and then in different biofilm formation process varied in E. coli. The experiment was aimed of providing a new theoretical basis for the treatment against calves diarrhea in clinical. Key words: Escherichia coli; biofilm; screening; growth curve 0 引言 pH 、铁离子浓度、氧化还原电位变化等对生物膜的形 [4] 细菌生物膜(Biofilm，BF)是相对于单个、分散的、 成具有重要作用 。生物膜可由纯菌种形成，也可以