白三烯b4及瞬时电位香草酸亚型受体1变化在急性胰腺炎中的作用.doc

PAGE PAGE 6 LTB4、TRPV1及CGRP在急性胆源性胰腺炎中的表达意义 夏炳杰1 陈思羽2 黄会云2 陈玉2 张涛2# （1桂林医学院附属医院 广西桂林54100；2广西中医药大学附属瑞康医院 南宁530011） 摘要：目的：通过检测白三烯B4（Leukotriene B4，LTB4）、瞬时电位香草酸亚型受体1（Transient Receptor Potential Vanilloid Channel-1，TRPV1）及降钙素基因相关肽（Calcitonin Gene Related Peptide，CGRP）等指标的变化，分析LTB4-TRPV1信号活化在急性胆源性胰腺炎中的作用。方法：取清洁级SD大鼠60只，随机分为正常组、模型组和TRPV1拮抗剂组。采用胆胰管开口十二指肠结扎法制备重症胰腺炎模型，术后3 h，除正常组外，其余两组开始分组给药，术后12 h，处死全部大鼠，腹主动脉取血并收集胰腺组织备测。用光镜检测胰腺组织超微结构变化，用ELISA技术检测LTB4变化，用Real-time PCR技术检测TRPV1、CGRP变化。结果：正常组胰腺呈浅粉红色；模型组见胰腺坏死、出血，部分可见腹水，腹水含血，光镜下见胰腺腺体结构紊乱，小叶间隔明显增大，腺体实质内可见大片凝固性坏死并伴有出血，坏死灶及周围有大量炎细胞浸润；TRPV1拮抗剂组胰腺组织充血水肿较模型组减轻，未见腹水，光镜下胰腺实质可见少许坏死、出血及少量炎性细胞浸润，其组织病理学评分较模型组下降，有统计学意义，P＜0.05。模型组见血淀粉酶、LTB4表达明显上升；TRPV1拮抗剂组血淀粉酶、LTB4表达下降，较模型组有统计学意义，P＜0.05；模型组胰腺组织TRPV1、CGRP mRNA表达上升；TRPV1拮抗剂组TRPV1、CGRP mRNA表达下降，与模型组比较有统计学意义，P＜0.05。结论：内源性LTB4活化TRPV1信号在胆道梗阻性胰腺炎中可能发挥至关重要的作用，阻断TRPV1信号转导将为急性胰腺炎的治疗提供新的思路。 关键词：急性胆源性胰腺炎；白三烯B4；瞬时电位香草酸亚型受体1；降钙素基因相关肽；表达 Expression and Significance of LTB4, TRPV1 and CGRP in Acute Biliary Pancreatitis XIA Bing-jie1, CHEN Si-yu2, HUANG Hui-yun2, CHEN Yu2, ZHANG Tao2# (1The Affiliated Hospital of Guilin Medical College, Guilin, Guangxi54100; 2The Affiliated Ruikang Hospital of Guangxi Abstract: Objective: Through the detection of the changes of leukotriene B4 (LTB4), transient receptor potential vanilloid channel-1 (TRPV1) and calcitonin gene related peptide (CGRP) to analyze the role of LTB4-TRPV1 signal activation in acute biliary pancreatitis. Methods: 60 SD rats were randomly divided into normal group, model group, and TRPV1 antagonist group. The acute pancreatitis model was established by biliary and pancreatic duct with the duodenum ligation. 3 hours after the surgery, the model group and TRPV1 antagonist group were given different drugs, except the normal group. 12 hours after the surgery, all rats were killed, took blood from the abdominal aorta and collected pancreatic tissue for measure. The changes of pancreatic tissue ultra structure were detected by light m