口服高氧液抗小鼠运动性疲劳作用与机制研究.doc

口服高氧液抗小鼠运动性疲劳的作用及机制研究 刘春然1,张国良1,徐礼鲜1 （第四军医大学:1口腔医院麻醉科，陕西 西安 710032） A experimental study of hyperoxia solution taken orally on fatigue and its mechanism in mice induced by exercise LIU Chun-Ran1, ZHANG Guo-Liang1, XU L i-Xian1 1Department of Anesthesiology,College of Stomatology, Fourth Military Medical University , Xi’an 710032 , China 【Abstract】AIM：To investigate the antifatigue effect of hyperoxia solution (HO) and its mechanism. METHODS：The mice were divided randomly into two groups: control group(C group) , hyperoxia solution group(HO group).Then each group was divided into three smaller groups by dose, which were infused with balanced salt solution or HO at 0.15ml、0.25ml and 0.5ml per 10g.After the mice were infused intragastricly, the contents of muscle glucogen, lactic acid and malondialdehyde，the general ability of antioxidation and the energy of adenosine triphosphate enzyme were determined instantly and after swimming 30 minute while they were observed about common state. RESULTS：Hyperoxia solution group swam more actively and sank fewer than control group. The contents of lactic acid of hyperoxia solution group(8.91~10.42±1.41 mmol/L) were smaller significantly than those of control group (11.91~12.01± 1.34mmol/L,P0.05);the content of muscle glucogen of hyperoxia solution group(1.57~2.07±0.15mg/g) was bigger distinctly than those of control group (1.03±0.14mg/g,P0.05) ; the activity of adenosine triphosphate enzyme of hyperoxia solution group(1.10~1.49±0.12μmolPi/mgprot/hour) increased more than those of control group(0.79~1.08±0.13μmolPi/mgprot/hour,P0.05);the content of malondialdehyde of hyperoxia solution group(17.74~18.93±1.26 nmol/ mgprot) were smaller significantly than those of control group(20.50~ 20.55±1.24nmol/mgprot,P0.05);the general ability of antioxidation of hyperoxia solution group(175.87~202.87±22.00nkat/mgprot )increased more than those of control group (134.53~137.36±16.00nkat/mgprot,P0.05). CONCLUSION:HO can increase moving capabi