桉木氨水浸渍预处理响应面优化提高酶解效率 - 中国桉树.pdf

第 30 卷 第 4 期 桉树科技 Vol.30 No.4 2013 年 12 月 EUCALYPT SCIENCE TECHNOLOGY Dec.2013 桉木氨水浸渍预处理响应面优化提高酶解效率 ＊ 霍 丹，房桂干 ，邓拥军，韩善明，沈奎忠 ( 中国林业科学研究院林产化学工业研究所，江苏 南京 210042) 摘要：采用氨水中温浸渍(SAA)方法对桉木进行预处理，以期提高其后续酶水解效率。以响应面法主要预处理条 件进行优化，以期筛选出 SAA 预处理的最优工艺条件。结果表明：最佳预处理工艺为氨用量 80%、时间 11 h、 温度 90℃，在此条件下预处理物料的后续酶解转化率可达 31.7% 。对预处理物料的分析表明：在 SAA 预处理过 程中，可在不损失纤维素的前提下脱除木素 51%，而且主要是紫丁香基型和愈疮木基型木素降解溶出，半纤维素 主要是木聚糖也有部分降解溶出。木素及半纤维素的降解、纤维素结晶度的降低都有利于后续的酶水解。 关键词：氨水；SAA 预处理；桉木；酶水解；还原糖 中图分类号：S781 文献标识码：A Pretreatment of Eucalypt Wood with Optimized SAA (Soaking in Aqueous Ammonia) for Conversion of Lignocellulose to Fermentable Sugars HUO Dan, FANG Gui-gan, DENG Yong-jun, HAN Shan-ming, SHEN Kui-zhong (Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing 210042, Jiangsu , China) Abstract: The methodology of soaking eucalypt wood in aqueous ammonia (SAA) as a pretreatment for enhancing production of fermentable sugars from enzymolysis process was investigated in this study. Various pretreatment conditions including temperature, time and dosage of aqueous-ammonia on the enzymatic digestibility were optimized using response surface methodology. An enzymatic digestibility of 31.7% was obtained when the eucalypt fibers were pretreated at the optimal conditions: 80.0% ammonia; temperature of 90°C; and, a duration of 11 h. The fourier transform infrared spectroscopy (FTIR) analysis indicated that hemicellulose degradation and lignin structure transformation occurred during SAA pretr