甲状腺功能亢进肝阳上亢证大鼠下丘脑组织蛋白质差异表达的观察.pdf

·18· Chinese Journal of Information on TCM May 2007 Vol.14 No.5 ·实验研究 · 甲状腺功能亢进肝阳上亢证大鼠下丘脑组织 蛋白质差异表达的观察 周凌燕,陈泽奇,李 炜,李 智,颜永平 (中南大学湘雅医院中西医结合研究所,湖南 长沙 410008) 摘要：目的 利用蛋白质组学方法,观察甲状腺功能亢进(简称“甲亢”)肝阳上亢证大鼠与正常大鼠下丘脑组织 的双向凝胶蛋白图谱,寻找差异表达的蛋白质。方法 左甲状腺素钠(L-T4)和附子汤灌胃造成甲亢肝阳上亢证模型, 应用蛋白质组学技术比较模型组与正常组中下丘脑的差异蛋白质,结合生物信息学对其进行分析鉴定。结果 两组蛋 白斑点总体分布相似,主要分布在等电点 pI 3～8,分子量 Mr 14.4～75 kD；分析发现,模型组与正常组相比有 18 个 蛋白质上调和 24 个蛋白质下调,进一步质谱鉴定,其中 7 个蛋白质分别为 NSFL1 辅助因子、硫氧还蛋白、泛素羧基 末端水解酶同工酶 L1、血小板活化因子乙酰基水解酶 IB-γ亚单位、谷氨酸脱氢酶前体、二氢嘧啶酶相关蛋白 2 和 微管蛋白。结论 模型组与正常组相比,有18个蛋白质点上调,有24个蛋白质点下调,经质谱鉴定了其中部分蛋白质。 蛋白质的变化可能与甲亢肝阳上亢证的形成密切相关。 关键词：甲状腺功能亢进；肝阳上亢证；下丘脑；蛋白质组学；动物模型 中图分类号：R289.11 文献标识码：A 文章编号：1005-5304(2007)05-0018-03 ZHOU Ling-yan, CHEN Ze-qi, LI Wei, et al (Chinese Traditional and Westenr Modern Medicine Research Institute of Xiangya Hospital, Central South University, Changsha 410008, China) Utilization of proteome method, by observing differences on protein expression of hypothalamus in the hyperthyroidism rats with hyperactivity of liver-yang and normal rats, to search the differential protein. Models of animal were prepared by ip L-T4 and Fuzi Decoction. Both the quantitative and qualitative changes of the protein expression were compared between model group and treated group by protein technique.Proteins were mainly displayed at range of pI 3～8, Mr (14.4～ 75)kD. 18 protein increased and 24 protein decreased in model group compared with treated group, included Thioredoxin, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Platelet-activating factor acetylhydrolase IB gamma subunit, glutamate dehydrogenase, dihydropyrimidinase-related protein 2, NSFL1 cofac