高效液相色谱法测定保健食品中他达拉非、西地那非、伐地那非违禁药物含量.pdfVIP

： L 堡笪塑 ___ L一_ C==l 同 效液相色谱法测定保健食品中他达拉非 西地那非 伐地那非违禁药物含量 沈志武 。唐宏兵 。李 群 。段晋仁。 (1．常州市疾病预防控制中心，常州213003；2．常州市卫生监督所，常州213003) 摘 要：应用高效液相色谱法测定了保健食品中他达拉非、西地那非、伐地那非等三种违禁药物 含量。采用ODS色谱柱，乙腈～0．05 mol·I 三乙胺(40+60)为流动相，流速 1．0 m1 ·min一 ，二 极管阵列检测器，检测波长290 nil3．。根据保留时间、光谱图定性，峰高或峰面积定量。该方法三种 药物组分在0．5～100．0 mg·L 浓度范围内呈线性关系，他达拉非和西地那非的检出限为 0．3 mg·I ～ ，伐地那非的检出限为0．4 mg·I 一 ，相对标准偏差为1．5 9／6～2．0 9／6，回收率为 95．8 9／6～107．0 9／6。 关键词：高效液相色谱法；保健食品；他达拉非；西地那非；伐地那非 中图分类号：0657．7 文献标识码：A 文章编号：1001—4020(2008)06—0540—03 HPLC Determination of 3 Contraband Drugs(Tadalafil，Sildenafil and VardenafiI)in Health-care Foodstuffs SHEN Zhi—Wil ，TANG Hong-bing ，LI Qun ，DUAN Jin-ren (1．Changzhou Municipal Center of Control and Prevention of Diseases，Changzhou 213003，China； 2．Changzhou Municipal Institute of Health Supervision，Changzhou 213003，China) Abstract：HPLC was applied tO the determination of 3 contraband drugs，i．e．，tadalafil，sildenafil and vardenafil，in health-care foodstuffs．Prodigy 5 ODS 3 column(250 rnmX4．6 mm)was used as stationary phase， and a mixture of acetonitrile and 0．05 mo1．L— triethylamine solution，mixed in the ratio of 40 tO 60，was used as mobile phase，with a flow-rate of 1．0 mL·min ．UV-deteetion at 290 nIn with diode array detector(DAD)wss adopted in the determination．Qualitative analysis was performed On the base of retention time and with reference to the standard spectra．Linear relationships between values of peak area and concentration of all the 3 drugs were found in the range of 0．5—100．0 mg·L～．Detection limits(S／N一3)found for tadalafil and sildenafil were 0．3 mg·L‘ ，and for vardenafil was 0．4 mg·L ．Precision and recovery were tested on known samples，and values of RSD S( 一6)found were in the ra