高温胁迫对不同基因型玉米雄穗保护酶活性的影响 - 玉米科学.doc

不同成熟度玉米叶片高温胁迫 (1.河南农业大学农学院, 郑州 450002; 2.河南粮食作物协同创新中心, 郑州 450002; 3.省部共建小麦玉米作物学国家重点实验室, 郑州 450002） 摘 要: 为探索营养生长与生殖生长并进时期玉米叶片抗氧化生理对高温胁迫的响应特征，以郑单958(ZD958)和先玉335(XY335)为试验材料，于第9片叶完全展开至抽雄期，每天830-17:30进行人工模拟增温处理，研究高温胁迫下玉米叶片膜透性、抗氧化酶活性以及渗透调节物质的动态变化。结果表明：(1)成熟玉米叶的相对电导率大于幼嫩叶，高温胁迫导致玉米叶片相对电导率增加，ZD958成熟叶的增加幅度大于幼嫩叶，XY335成熟叶的增加幅度小于幼嫩叶(2)高温成熟玉米叶的大于幼嫩叶，高温胁迫导致玉米叶片增加，ZD958成熟叶的增加幅度大于幼嫩叶，XY335成熟叶的增加幅度小于幼嫩叶(4)与对照相比较，高温胁迫下，可溶性蛋白无显著性变化成熟叶可溶性糖含量呈趋势在XY335幼嫩叶；游离性脯氨酸在成熟叶中呈降-升-降，幼嫩叶呈趋势幼嫩叶。不同品种不同成熟度叶片对高温的敏感性不同。XY335敏感性ZD958，ZD958成熟叶敏感性幼嫩叶，XY335幼嫩叶敏感性成熟叶。玉米；高温胁迫；叶片；抗氧化esponses of antioxidative physiology to high temperature in different maturity leaves of maize SUN Ning-ning1, YU Kang-ke1, ZHAN Jing1, GU Hai-jing1, PAN Li-wen1, LIU Gang1, LIU Tian-xue1,2,3* (1. Agronomy College, Henan Agricultural University, Zhengzhou 450002; 2.State Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 450002; 3.Collaborative Innovation Center of Henan Grain Crops, Zhengzhou 450002, China) Abstract: In order to explore response of antioxidative physiology to high temperature in maize leaf during the growth progress of vegetative and reproductive, using two maize hybrids of Zhengdan958(ZD958) and Xianyu335(XY335), from the 9th leaf fully expanded to tasseling stage, plants were given a high temperature treatment(T) from 8:30 to 17:30 daily in an artificial simulation heat device, studying the dynamics of membrane permeability, activity of antioxidant enzymes and osmotic regulation substances of maize leaf under heat stress. The results show that: (1)The relative electrical conductivity of mature leaves is higher than the young leaves and increasing during high temperature stress, the increase extent was higher in mature leaves than in young leaves of ZD958, while it was lower in mature leaves than in young leaves of XY335. (2) The superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) show different trends among maize varieties and leaves under heat stress. In the early stage of high tempera