浅谈粉防己碱对人膀胱移行细胞癌BIU-87细胞裸鼠移植瘤的实验.PDFVIP

粉防己碱对人膀胱移行细胞癌BIU-87 细胞裸鼠移植瘤的实验研究 李永生1 2 2 ，赵诚 ，胡钊 (1.福建医科大学附属协和医院泌尿外科，福州 350001 ；2.福建医科大学协和临床医学院泌尿外科，福州 350001) 摘要：目的 研究粉防己碱(TET)对人膀胱移行细胞癌BIU-87 细胞株裸鼠移植瘤的作用机制。方法 14 只裸鼠右后腿背 侧皮下接种BIU-87 移植瘤细胞后随机分为磷酸盐缓冲液(PBS)对照组(7 只)和25 μg·mL1 TET 治疗组(7 只) 。接种1 个月 后开始，每3 d 一次于肿瘤局部注射给予PBS 或TET ，每3 d 测量1 次肿瘤大小；RT-PCR 检测Bcl-2 、Bax mRNA 表达； Western-blot 分析survivin、Capseae-3 蛋白表达。结果 TET 对人膀胱癌BIU-87 细胞裸鼠移植瘤的生长具有抑制作用， 抑制率达66.6%。RT-PCR 检测结果显示，与PBS 对照组相比，TET 能明显下调移植瘤Bcl-2 mRNA 的表达，上调Bax mRNA 的表达(P0.05) ；Western-blot 结果显示经 TET 处理后 survivin 蛋白出现明显下调，而 Capseae-3 蛋白的表达上调明显 (P0.05) 。结论 TET 能够抑制人膀胱癌BIU-87 细胞裸鼠移植瘤的生长，其作用的分子机制与下调Bcl-2 、survivin 和上 调Bax 、Capseae-3 基因表达有关。 关键词：粉防己碱；膀胱癌；Bcl-2 ；Bax ；survivin ；Capseae-3 中图分类号：R285.5 文献标志码：A 文章编号：1007-7693(2012)12-1070-04 Effect of TET on Xenografted Tumor of Human Bladder Cancer BIU-87 Cells on Nude Mice 1 2 2 LI Yongsheng , ZHAO Cheng , HU Zhao (1.Department of Urological Surgery, Union Hospital Affiliated to Fujian Medical University, Fuzhou 350001, China; 2.Department of Urological Surgery, Union Clinical College of Fujian Medical University, Fuzhou 350001, China) ABSTRACT: OBJECTIVE To investigate the effects of tetrandrine(TET) on the xenografted tumor of the human bladder cancer BIU-87 cell line on nude mice. METHODS Fourteen nude mice which had been inoculated with BIU-87 cells by subcutaneous injection into the right legs were divided into the PBS control group(7 mice) and the 25 μg·mL1 TET group(7 mice). After a month of transplantating tumor cell, mice were given PBS or TET into the tumor once every 3 days. Tumor volumes were measured every 3 days. RT-PCR was used to detect Bcl-2 and Bax mRNA expressions. The expressio