流式软件 flowjo教程 基础.pdf

Flowjo Data Analysis Software for Flow Cytometry Version X User Documentation Windows/Mac user documentation Basic Tutorial FlowJo was written by Adam Treister and Mario Roederer beginning in 1996, based on concepts developed at the Herzenberg Laboratory at Stanford. We are indebted to our active and enthusiastic users worldwide for their ideas, discussions and tireless testing of new versions. FlowJo, its tutorials, documentation and web site are copyright © Tree Star, Inc. 1997-2013. All rights reserved. • Basic Tutorial • • © MMXIII • Revision date: 16 April 2013 version 10.0.6 i Table of Contents Introduction iii Getting Started 1 Lesson 1 - The Workspace 3 Lesson 2 - Gating 7 Lesson 3 - Statistics 10 Lesson 4 - Batching in the Workspace 12 Lesson 5 - Basic Table Editor Functionality 14 Lesson 6 - Basic Layout Editor Functionality 18 Saving25 ii Introduction - FlowJo Basic Tutorial FlowJo is an integrated environment for viewing and analyzing flow cytometric data, presented in the form of a Workspace. The Workspace contains a list of all of the data samples that you load, the gates, statistics and other analyses that you apply, and the table and graphical layout that you design. The Workspace is saved as a FlowJo document on your hard disk; when you reopen the document, you will see the status of your analysis as it was when it was last saved. This tut