文拉法辛对卒中后抑郁大鼠学习记忆及海马脑源性神经营养因子的影响2011年.docVIP

文拉法辛对卒中后抑郁大鼠学习记忆及海马脑源性神经营养因子的影响 [摘要] 目的：观察盐酸文拉法辛对卒中后抑郁(post-stroke depression, PSD)大鼠学习记忆障碍的改善作用，探讨盐酸文拉法辛对认知的改善与海马CA3区脑源性神经营养因子(brain-derived neurotrophic factor, BDNF)表达变化的关系。方法：50只成年雄性SD大鼠随机分对照组、模型组及3个治疗剂量组(510、20 mg.kg-1)，每组10只。采用选择性右侧大脑中动脉栓塞连续3周的慢性不可预见性温和应激方法建立卒中后抑郁大鼠模型，在慢性应激处理的同时，不同剂量的文拉法辛每日次在固定的时间段腹腔注射入PSD大鼠。用Morris水迷宫试验评价空间学习与记忆功能，用免疫组织化学方法分析海马CA3区BDNF的表达。 结果：与对照组比较，模型组大鼠学习能力显著下降(P0.05)，反映记忆功能的空间探索时间和跨平台次数均显著低于对照组，分别为(14.2±4.8 vs (45.9±4.5 ) s及(1.3±0.3 次 vs 8.3±1.1) 次而且BDNF阳性细胞数也较对照组显著下降(9.8±3.2 vs 18.5±4.7)，差异具有统计学意义(P0.05)。文拉法辛510及20 mg.kg-1.d-1治疗，PSD大鼠认知功能BDNF表达显著提高结论：文拉法辛改善卒中后抑郁大鼠学习记忆障碍，可能与增加海马脑源性神经营养因子有关。 [关键词] 海马环己醇类投药和剂量卒中抑郁症脑源性神经营养因子记忆障碍随机对照试验 [中图分类号] R74 [文献标码] A [文章编号]1008-9292（2011）ffect of venlafaxine on cognitive function and hippocampal brain-derived neurotrophic factor expression in rats with post-stroke depression DAI Mu-hua, LI De-qiang, HAN Yang (The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China) [Abstract] Objective: To evaluate the effect of venlafaxine on the cognitive impairment of learning and memory in rats with post-stroke depression (PSD) and to investigate its relationship with the expression of brain-derived neurotrophic factor (BDNF) in hippocampus. Methods: Fifty male adult SD rats were randomly divided into control group, model group and three treatment groups (5, 10, 20 mg.kg-1 venlafaxine) with ten in each group. After the procedure of selective cerebral right middle artery embolism, a paradigm of continuous 3-week chronic unpredictable mild stress (CUMS) was used to induce PSD. Along with the course of CUMS and peritoneal injection at different dose levels of venlafaxine were performed once a day in PSD rats in a fixed time interval. Morris water maze test was applied to assess the spatial learning and memory function and immunohistochemical staining was used to detect the change of BDNF expression. Results: The learning function decreased significantly in PSD rats com