大鼠急、慢性毒鼠强中毒模型的建立.pdfVIP

大鼠急、慢性毒鼠强中毒模型的建立* 1，2 1 1 1 陈伟杰 ，陶 涛 ，李 斌 ，廖志钢 1.四川大学华西基础医学与法医学院，四川成都 610041； 2. 浙江省余姚市公安局，浙江余姚315400；） 摘要 研究背景及目的：毒鼠强是一种剧杀鼠药，对人、畜危害极大，临床诊治和法医学鉴定 经常遇到毒鼠强中毒的案例，但是对毒鼠强中毒的机制仍不明确。为了对研究毒鼠强中毒大鼠脑内 GABA 及GABA R- 1 表达情况和变化规律提供动物模型。方 法：健康Sprague-Dawley 大鼠（S-D A 大鼠）60 只，随机分为正常对照组、空白对照组、急性中毒组和慢性中毒组，其中慢性中毒组按中 毒后处死时间不同分成30min，3h，6h，12h，24h，3d，5d，7d，10d 组，每组5 只。急性中毒组每 只以1.0mg/kg 的剂量用毒鼠强悬浊液灌胃，慢性中毒组每只以0.1mg/kg 的剂量用毒鼠强溶液灌胃制 作中毒模型。 结 果：①抽搐是毒鼠强中毒的主要临床表现；②病理学改变主要为蛛网膜下腔出血 和脑水肿；③根据大体形态和组织学改变，动物模型成功，为后期研究打下了基础。 [关键词] 毒鼠强; 中毒; 动物模型 An established experimental model of rats poisoned by tetramine 1，2 1 1 1 Chen Wei-j ie , Tao Tao , Li Bin , Liao Zhi-gang ( 1.School of West China Preclinical and Forensic Medicine, Sichuan University. Chengdu 6 1004 1. 2 The Public Security of Yuyao,Zhej iang province, yuyao 315400 ) [Abstract] Background and purpose Tetramine is a severe toxicant for killing mouse. Accidental and homicidal poisoning are common in both clinical and forensic aspects in China. But the intoxicated mechanism of tetramine is not known so far. To approach the intoxicated mechanisms we designed the experimental model of rat poisoned by tetramine and for later to study immunohistochemistry.expression of GABA and GABA R- 1. A Methods Sixty healthy Spragwe-Dawley rats were randomly divided into four groups including the control, the sham poisoned, the acute poisoned and the chr onic poisoned. And the latter was once more subdivided into nine subgroups according to the sacrificed time of animals at 30min, 3h, 6h, 12h, 1d, 3d, 5d, 7d