马铃薯东农303对两种抗生素敏感性的研究.pdfVIP

安徽农业科学，Joumal 0fAtfimi A ．Sci．2008，36(16)：6669—6670 责任编辑 罗芸 责任校对 李洪 马铃薯东农303对两种抗生素敏感性的研究 王萍 ，潘亚仙 ，王罡2，季静2，吕文河 (1．淮海工学院海洋学院，江苏连云港 222005；2．天津大学农业与生物工程学院，天津 300072；3．东北农业大学农学院，黑龙江哈尔滨 150030) 摘要 『目的]探讨马铃著东衣303对两种抗生素敏感性，为进一步改良与利用东衣303提供依据。[方法]以马铃著东农303无菌苗的无 腋芽茎段和单芽茎段为外植体，研究东衣303在诱导愈伤组织和芽的过程中对卡那霉素或头孢霉素的敏感性。[结果]东衣303无腋芽 茎段对两种抗生素的敏感性存在一定差异，东衣303以卡那霉素作为无腋芽茎段诱导愈伤组织的抗性筛选时适宜浓度为50 m#L，作为 单芽茎段芽生长的抗性筛选时适宜浓度为100 n~／I 。东农303对头孢霉素也较敏感。无腋芽茎段在含头孢霉素100 mg／L的培养基中 培养时芽的诱导率受到明显抑制。[结论]东农303对两种抗生素均较为敏感，对头孢霉素的抗性要高于卡那霉素。 关键词 马铃薯；茎段；卡那霉素；头孢霉素 中图分类号 $532 文献标识码 A 文章编号 0517—661l(2OO8)16—06669—02 Study on Sensibility ofthe PotatoCultivarDongnong 303toTwo Kinds ofAntibiotics 、 NG PiIlg et al (School of Marine Scienee and 3~hology，Huaihai Institute of Technology，Lianyungang，Jiangsu 222O∞) Abstract I Obiex：tive l 111e purpo~of this research was to investigate the sensibility of the potat()cuhivar Dongnong 303 to 2 antibiotics of kanamycin and eefot~xime and provide 1)ase for further modifying and utilizitrg ev．Dongnong 303．1 Methodl With stem without axilla bud and stem with single bud Oll a．~ptie seedling of 1)ongnong 303 as explant．the sensibility ofthe potato DongrlOllg 303 to kanamycin and eefotaxime during induction of cNlus and buds wos studye(1．j Resuit l rI11e sensibility ofstenl without axilla bud in Dongnong 303 had sortie difiefence to these two antibiotics．When kanamycin was即． D1ied as a resistant~leetion agent，the idea1 conen．ofkanamycin for the stem without axilla bud to induee callus ws 50 mg／L and that for the stenl with sin~e bud to induce shoot in Dongnong 303 was 100 nag mg／L．Dongnong 303 was also sensitive to cefotaxime．_丌1e induetion rate of the 1)uds was ie． strained significantly when the stem without axil1a bud was cultured in medium with eefotaxime at l00．g／I ．j Conclusion l Dongnong 303 was~nsitive to these two antibiotics and its resistance to eefotaxime was higher thml that to kmmmyein． Ke