用绿色荧光蛋白标记的细菌研究鱼体吸收颗粒抗原的部位 - 水产学报.PDF

24 5 Vol.24, No.5 2000 10 JOURNAL OF FISH RI S OF CHINA Oct.,2000 : 1000- 0615(2000) 05- 0472- 04 陈 营, 陆承平 (, 210095) GFP : 4332 (Ah4332 ) , ,, , , , , 60min , 10min30min 120min : ; ;; : S942.5 :A Application of green fluorescent protein marked bacteria to investigate the site of antigen uptake in Carassius auratus ibelio CH N Ying, LU Chengping (Key Lab of A nimal Disease Diag nostic and Immunology , Ministry of Agriculture, Nanj ing Agric Univ, Nanj ing 210095, China Abstract:The uptake of granular antigen was examined in Carassius auratus ibelio after bath administration of GFP GFPmarked Aeromonas hydrop hila strain 4332( Ah4332 ) by hyperosmotic infiltration ( HI) and direct immersion (DI) . Much more marked bacteria had been found in gills than that in skin and gut in both treatments throughout the experiment. Quantitative analyses indicated that the main site of antigen uptake were the gills in bothHI and DI treatments. In addition, the amount of marked bacteria in gills and skin had increased obviously after hyperosmotic treatment, and gut also had increased bacteria during experiment of 10min, 30min and 120min with the exception of 60 min after immersion. It showed that hyperosmotic treatment facilitated antigen entrance in fish. Key words: antigen uptake; green fluorescent protein; A eromonas hydrop hila; Carassius auratus ibelio [ 1] , , , , (green fluorescent protein, GFP) [ 2] GFP ,